{"title":"金龟子绿僵菌CQMa421与溴氰菊酯的协同作用提高了对背小实蝇的杀虫能力。","authors":"Jinyuan Zhao, Chen Shen, Yangjunlu Shen, Lijuan Liu, Wenkai Yan, Yujia Guan, Renwen Zheng, Sihan Lu, Qingfeng Tang","doi":"10.1017/S0007485325100217","DOIUrl":null,"url":null,"abstract":"<p><p><i>Bactrocera dorsalis</i> (Diptera: Tephritidae) is a highly invasive and destructive quarantine pests worldwide. To improved biological control efficiency, reduce chemical pesticides use, and optimise the application of <i>Metarhizium anisopliae</i> (Hypocreales: Clavicipitaceae) against <i>B. dorsalis</i>. This study evaluated the combined toxicity of <i>M. anisopliae</i> with deltamethrin and chlorpyrifos. The biocompatibility of <i>M. anisopliae</i> CQMa421 with these pesticides was assessed based on spore germination, mycelial growth, and sporulation. Additionally, the effects of combined treatments on detoxification enzyme and related gene expression in <i>B. dorsalis</i> were investigated. The results indicated that the virulence effect of <i>M. anisopliae</i> CQMa421 against <i>B. dorsalis</i> adults was time-dependent and dose-dependent. Deltamethrin showed good compatibility with <i>M. anisopliae</i> CQMa421, achieving 100% mortality at 1 × 10⁸ CFU/mL by 84 hours. Different concentrations of deltamethrin can promote the mycelial growth and sporulation of <i>M. anisopliae</i> CQMa421. The toxicity effect of deltamethrin and chlorpyrifos combined with <i>M. anisopliae</i> CQMa421 on <i>B. dorsalis</i> adults was better than that of single-agent treatment, and the co-toxicity factor of 5 mg/L deltamethrin and 1 × 10<sup>8</sup> CFU/mL <i>M. anisopliae</i> CQMa421 was 24.81, which synergistically affected on <i>B. dorsalis</i> control. Enzyme activity assays and qRT-PCR results revealed that the combination treatment differentially activated and enhanced the activities of AChE, CarE, GST, CAT, and SOD. Meanwhile, <i>BdCarE</i> was significantly inhibited and upregulating <i>BdGSTD7, BdGSTS1, BdCYP4ae</i>1, <i>BdPOD, BdPOD1</i>, and <i>BdCAT</i> genes. In conclusion, the combination of deltamethrin and <i>M. anisopliae</i> CQMa421 enhanced the insecticidal efficacy against <i>B. dorsalis</i>, significantly affected the activity of related detoxification enzymes. Provided a robust basis for integrating biological and chemical control strategies to manage <i>B. dorsalis</i> more effectively.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The synergistic effect of <i>Metarhizium anisopliae</i> CQMa421 and deltamethrin improved the insecticidal ability against to <i>Bactrocera dorsalis</i>.\",\"authors\":\"Jinyuan Zhao, Chen Shen, Yangjunlu Shen, Lijuan Liu, Wenkai Yan, Yujia Guan, Renwen Zheng, Sihan Lu, Qingfeng Tang\",\"doi\":\"10.1017/S0007485325100217\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Bactrocera dorsalis</i> (Diptera: Tephritidae) is a highly invasive and destructive quarantine pests worldwide. To improved biological control efficiency, reduce chemical pesticides use, and optimise the application of <i>Metarhizium anisopliae</i> (Hypocreales: Clavicipitaceae) against <i>B. dorsalis</i>. This study evaluated the combined toxicity of <i>M. anisopliae</i> with deltamethrin and chlorpyrifos. The biocompatibility of <i>M. anisopliae</i> CQMa421 with these pesticides was assessed based on spore germination, mycelial growth, and sporulation. Additionally, the effects of combined treatments on detoxification enzyme and related gene expression in <i>B. dorsalis</i> were investigated. The results indicated that the virulence effect of <i>M. anisopliae</i> CQMa421 against <i>B. dorsalis</i> adults was time-dependent and dose-dependent. Deltamethrin showed good compatibility with <i>M. anisopliae</i> CQMa421, achieving 100% mortality at 1 × 10⁸ CFU/mL by 84 hours. Different concentrations of deltamethrin can promote the mycelial growth and sporulation of <i>M. anisopliae</i> CQMa421. The toxicity effect of deltamethrin and chlorpyrifos combined with <i>M. anisopliae</i> CQMa421 on <i>B. dorsalis</i> adults was better than that of single-agent treatment, and the co-toxicity factor of 5 mg/L deltamethrin and 1 × 10<sup>8</sup> CFU/mL <i>M. anisopliae</i> CQMa421 was 24.81, which synergistically affected on <i>B. dorsalis</i> control. Enzyme activity assays and qRT-PCR results revealed that the combination treatment differentially activated and enhanced the activities of AChE, CarE, GST, CAT, and SOD. Meanwhile, <i>BdCarE</i> was significantly inhibited and upregulating <i>BdGSTD7, BdGSTS1, BdCYP4ae</i>1, <i>BdPOD, BdPOD1</i>, and <i>BdCAT</i> genes. In conclusion, the combination of deltamethrin and <i>M. anisopliae</i> CQMa421 enhanced the insecticidal efficacy against <i>B. dorsalis</i>, significantly affected the activity of related detoxification enzymes. Provided a robust basis for integrating biological and chemical control strategies to manage <i>B. dorsalis</i> more effectively.</p>\",\"PeriodicalId\":9370,\"journal\":{\"name\":\"Bulletin of Entomological Research\",\"volume\":\" \",\"pages\":\"1-9\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Entomological Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1017/S0007485325100217\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0007485325100217","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
The synergistic effect of Metarhizium anisopliae CQMa421 and deltamethrin improved the insecticidal ability against to Bactrocera dorsalis.
Bactrocera dorsalis (Diptera: Tephritidae) is a highly invasive and destructive quarantine pests worldwide. To improved biological control efficiency, reduce chemical pesticides use, and optimise the application of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) against B. dorsalis. This study evaluated the combined toxicity of M. anisopliae with deltamethrin and chlorpyrifos. The biocompatibility of M. anisopliae CQMa421 with these pesticides was assessed based on spore germination, mycelial growth, and sporulation. Additionally, the effects of combined treatments on detoxification enzyme and related gene expression in B. dorsalis were investigated. The results indicated that the virulence effect of M. anisopliae CQMa421 against B. dorsalis adults was time-dependent and dose-dependent. Deltamethrin showed good compatibility with M. anisopliae CQMa421, achieving 100% mortality at 1 × 10⁸ CFU/mL by 84 hours. Different concentrations of deltamethrin can promote the mycelial growth and sporulation of M. anisopliae CQMa421. The toxicity effect of deltamethrin and chlorpyrifos combined with M. anisopliae CQMa421 on B. dorsalis adults was better than that of single-agent treatment, and the co-toxicity factor of 5 mg/L deltamethrin and 1 × 108 CFU/mL M. anisopliae CQMa421 was 24.81, which synergistically affected on B. dorsalis control. Enzyme activity assays and qRT-PCR results revealed that the combination treatment differentially activated and enhanced the activities of AChE, CarE, GST, CAT, and SOD. Meanwhile, BdCarE was significantly inhibited and upregulating BdGSTD7, BdGSTS1, BdCYP4ae1, BdPOD, BdPOD1, and BdCAT genes. In conclusion, the combination of deltamethrin and M. anisopliae CQMa421 enhanced the insecticidal efficacy against B. dorsalis, significantly affected the activity of related detoxification enzymes. Provided a robust basis for integrating biological and chemical control strategies to manage B. dorsalis more effectively.
期刊介绍：
Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.
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