再生牙科临床应用牙干细胞的生产和生物银行:当前实践和未来展望。

IF 4.8 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Shuntaro Yamada , Christoph Malkmus , Elise Aasebø , Kamal Mustafa , Hiroshi Egusa , Ana Angelova Volponi
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引用次数: 0

摘要

目的:本综述的目的是绘制牙科干细胞制造实践和临床使用生物库的现状,并确定可能阻碍牙科干细胞治疗临床转化和标准化的可变因素。数据来源:使用Medline/PubMed, Web of Science和Scopus进行电子检索,以“Dental stem cells”和“Dental pulp stem cells”为关键词。此外,ClinicalTrials.gov还用于确定用于治疗目的的牙科干细胞的临床试验。我们使用了相关的文章和参考书目来进行叙述回顾。研究选择:最初的搜索从PubMed获得48条记录,从Scopus获得66条记录,从Web of Science获得33条记录,从ClinicalTrials.gov获得23项注册试验。总共确定了23项注册试验,其中14项涉及在临床环境中使用牙科干细胞进行治疗。为了回顾目前牙科干细胞制造用于临床的实践,我们选择了21项研究进行深入的全文分析,其中5项研究涉及含有牙科干细胞的微移植物的椅侧制备。结论:尽管牙科干细胞已经应用于临床再生治疗,但细胞制造实践在报道的病例中差异很大,反映出缺乏标准化。此外,牙科特定的挑战,如后勤限制和多个利益相关者的复杂参与,阻碍了严格的监管要求的遵守,与其他领域的体细胞治疗药物相比,导致临床转化较慢。这凸显了学术界、产业界、临床医生和监管机构之间需要进行全球合作,以建立标准化实践,增强可预测性,并确保长期可追溯性。临床意义:牙科干细胞已在临床环境中使用,私人医生在将其转化为牙科再生疗法方面发挥着主导作用。随着越来越多的企业进入全球牙科干细胞生物银行市场,这一趋势预计将加速。目前,不同组织的细胞制造协议差异很大,应用了复杂的监管框架。此外,关于长期冷冻保存牙齿干细胞的使用和此类产品的调节解释仍然存在不确定性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production and biobanking of dental stem cells for clinical applications in regenerative dentistry: Current practices and future perspectives—A narrative review

Objectives

The aim of the review was to map the current landscape of dental stem cell manufacturing practice and biobanking for clinical use and to identify variable factors that may hinder clinical translation and standardization of dental stem cell-based therapy.

Data sources

An electronic search was performed using Medline/PubMed, Web of Science, and Scopus, using “Dental stem cells” and “Dental pulp stem cells” as keywords. In addition, ClinicalTrials.gov was used to identify clinical trials of dental stem cells for therapeutic purposes. Relevant articles and bibliographic references from the reviewed studies were used to develop the narrative review.

Study selection

The initial search yielded 48 records from PubMed, 66 from Scopus, 33 from Web of Science, and 23 registered trials from ClinicalTrials.gov. In total, 23 registered trials were identified, of which 14 involved the therapeutic use of dental stem cells in clinical settings. For reviewing the current practices of dental stem cell manufacturing for clinical use, 20 studies were selected for an in-depth full-text analysis, among which fifteen and five articles addressed the ex-vivo expanding cells and chair-side preparation of micrograft containing dental stem cells, respectively.

Conclusion

Although dental stem cells have been applied in clinical settings for regenerative therapies, cell manufacturing practices have varied widely across reported cases, reflecting a lack of standardization. Furthermore, dental-specific challenges—such as logistical constraints and the complex involvement of multiple stakeholders—hinder compliance with stringent regulatory requirements, contributing to slower clinical translation compared to somatic-cell therapy medicine in other fields. This highlights a need for global collaboration among academia, industry, clinicians, and regulators to establish standardized practice, enhance predictability, and ensure long-term traceability.

Clinical significance

Dental stem cells have been used in clinical settings, with private practitioners playing a leading role in translating them into regenerative therapies in dentistry. This trend is expected to accelerate, driven by the growing number of enterprises entering the global dental stem cell biobanking market. Currently, cell manufacturing protocols vary widely across organizations with intricated regulatory framework applied. Furthermore, uncertainties remain regarding the use of long-term cryopreserved dental stem cells and the regulatory interpretation of such products.
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来源期刊
Journal of dentistry
Journal of dentistry 医学-牙科与口腔外科
CiteScore
7.30
自引率
11.40%
发文量
349
审稿时长
35 days
期刊介绍: The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.
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