Willis Kwun Hei Ho, Qin Zhang, Fariza Zhorabe, Jiaxiang Yan, Yutian Gu, Shujun Wang, Changqing Yi, Yu Zhang, Mo Yang
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引用次数: 0
摘要
淀粉样蛋白-β (a β)斑块是阿尔茨海默病(AD)的一个关键病理标志,因此需要高灵敏度的a β生物检测方法来诊断AD。在这里,我们合成了一种浮力等离子体衬底,由聚乙烯醇微泡(mb)组成,表面装饰有原位还原金纳米颗粒(Au NPs)。得益于其固有的浮力和近红外等离子体特性,Au/MB衬底通过表面增强拉曼光谱(SERS)技术成为生物分子传感的理想平台。与传统的平面SERS衬底相比,Au/MB衬底的三维(3D)曲面显著增加了有效传感面积,同时其固有的浮力有利于有效去除未结合的目标,从而提高了检测特异性。通过铜离子(Cu2+)和4-巯基苯甲酸(4-MBA)功能化Au/MB底物,我们实现了ad相关Aβ蛋白的灵敏检测。在目标分析物存在的情况下,Aβ蛋白与Cu2+的相互作用引起4-MBA分子的变形和取向变化,导致SERS信号发生明显的光谱变化。结果表明,基于Au/ mb的SERS传感器可以灵敏地检测a β1-40低聚物,灵敏度低至10-9 m。因此,本工作不仅为设计基于浮力等离子体底物的SERS传感平台奠定了基础框架,而且为疾病相关蛋白生物标志物的定量检测铺平了道路,有助于AD诊断的进步。
A buoyant plasmonic microbubble-based SERS sensing platform for amyloid-beta protein detection in Alzheimer's disease.
Amyloid-β (Aβ) plaques are a key pathological hallmark of Alzheimer's disease (AD), highlighting the need for highly sensitive bioassays for Aβ detection to enable AD diagnosis. Here, we synthesized a buoyant plasmonic substrate composed of polyvinyl alcohol microbubbles (MBs) decorated with in situ-reduced gold nanoparticles (Au NPs). Benefiting from its inherent buoyancy and near-infrared plasmonic properties, the Au/MB substrate serves as an ideal platform for biomolecular sensing via the surface-enhanced Raman spectroscopy (SERS) technique. Compared to conventional flat SERS substrates, the three-dimensional (3D) curved surface of the Au/MB substrate significantly increases the effective sensing area, while its inherent buoyancy facilitates the efficient removal of unbound targets, thereby enhancing detection specificity. By functionalizing Au/MB substrates with copper ions (Cu2+) and 4-mercaptobenzoic acid (4-MBA), we achieved sensitive detection of AD-related Aβ proteins. In the presence of the target analyte, the interaction between Aβ proteins and Cu2+ induces molecular deformation and orientation changes in 4-MBA, leading to distinct spectral changes in the SERS signals. The results demonstrate that the developed Au/MB-based SERS sensor enables sensitive detection of Aβ1-40 oligomers with a sensitivity as low as 10-9 M. Therefore, this work not only establishes a foundational framework for designing buoyant plasmonic substrate-based SERS sensing platform but also paves the way for the quantitative detection of disease-associated protein biomarkers, contributing to advancements in AD diagnostics.