{"title":"黄洋参功能盐的生化特性、抗氧化和抗增殖试验:超声和温度制度的影响","authors":"Bhagirath Rakhasiya , Digvijay Singh Yadav , Siddharth H. Jasani , Deepesh Khandwal , Rajendra Singh Thakur , Kamalesh Prasad , Avinash Mishra , Vaibhav A. Mantri","doi":"10.1016/j.foohum.2025.100684","DOIUrl":null,"url":null,"abstract":"<div><div>This study investigates the effects of sonication and temperature on the extraction and biological activities of oligosaccharide-rich salts derived from the red seaweed <em>Gracilaria corticata</em>. Time (0, 5, 10, and 15 min) and temperature (10, 20, 30, and 40 °C) were varied to assess their impact on yield, antioxidant activity, mineral composition, cytotoxicity, and potential anticancer activity in Chinese hamster ovary (CHO) and hepatocyte-derived carcinoma (Huh-7) cell lines. The highest yield (51.34 ± 2.21 %) was obtained at 10 °C with 10 min of sonication, while the highest antioxidant activity (1.09 ± 0.12 mg g<sup>−1</sup>) was observed at 20 °C with 15 min of sonication. Mineral analysis showed that potassium was 64707.00 ± 53597.41 mg kg<sup>−1</sup> at 10°C, 0-minute, and iron at 144.86 ± 48.06 mg kg<sup>−1</sup> at 20°C, 0-minute. NMR analysis revealed the highest galactose content (46.16 ± 7.63 %) in the yield obtained at 20 °C with 0 min of sonication. FT-IR confirmed that temperature and sonication affected the rheological properties of agar. Biological assays showed anti-proliferative activity of 37.99 ± 8.70 % on Huh-7 and 38.59 ± 6.10 % on CHO cell lines (20°C, 0-minute), lowest wound healing 21.42 % and 26.49 % after 6 and 12 h (20°C, 0-minute). This is the first study that optimized temperature sonication enhances the yield and bioactivity of <em>Gracilaria corticata</em> salts, offering a scalable route to nutraceutical and pharmaceutical applications.</div></div>","PeriodicalId":100543,"journal":{"name":"Food and Humanity","volume":"5 ","pages":"Article 100684"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biochemical characterization, antioxidant and antiproliferative assays of functional salt derived from Gracilaria corticata: Effects of ultrasound and temperature regimes\",\"authors\":\"Bhagirath Rakhasiya , Digvijay Singh Yadav , Siddharth H. Jasani , Deepesh Khandwal , Rajendra Singh Thakur , Kamalesh Prasad , Avinash Mishra , Vaibhav A. Mantri\",\"doi\":\"10.1016/j.foohum.2025.100684\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study investigates the effects of sonication and temperature on the extraction and biological activities of oligosaccharide-rich salts derived from the red seaweed <em>Gracilaria corticata</em>. Time (0, 5, 10, and 15 min) and temperature (10, 20, 30, and 40 °C) were varied to assess their impact on yield, antioxidant activity, mineral composition, cytotoxicity, and potential anticancer activity in Chinese hamster ovary (CHO) and hepatocyte-derived carcinoma (Huh-7) cell lines. The highest yield (51.34 ± 2.21 %) was obtained at 10 °C with 10 min of sonication, while the highest antioxidant activity (1.09 ± 0.12 mg g<sup>−1</sup>) was observed at 20 °C with 15 min of sonication. Mineral analysis showed that potassium was 64707.00 ± 53597.41 mg kg<sup>−1</sup> at 10°C, 0-minute, and iron at 144.86 ± 48.06 mg kg<sup>−1</sup> at 20°C, 0-minute. NMR analysis revealed the highest galactose content (46.16 ± 7.63 %) in the yield obtained at 20 °C with 0 min of sonication. FT-IR confirmed that temperature and sonication affected the rheological properties of agar. Biological assays showed anti-proliferative activity of 37.99 ± 8.70 % on Huh-7 and 38.59 ± 6.10 % on CHO cell lines (20°C, 0-minute), lowest wound healing 21.42 % and 26.49 % after 6 and 12 h (20°C, 0-minute). This is the first study that optimized temperature sonication enhances the yield and bioactivity of <em>Gracilaria corticata</em> salts, offering a scalable route to nutraceutical and pharmaceutical applications.</div></div>\",\"PeriodicalId\":100543,\"journal\":{\"name\":\"Food and Humanity\",\"volume\":\"5 \",\"pages\":\"Article 100684\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food and Humanity\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2949824425001880\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Humanity","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2949824425001880","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Biochemical characterization, antioxidant and antiproliferative assays of functional salt derived from Gracilaria corticata: Effects of ultrasound and temperature regimes
This study investigates the effects of sonication and temperature on the extraction and biological activities of oligosaccharide-rich salts derived from the red seaweed Gracilaria corticata. Time (0, 5, 10, and 15 min) and temperature (10, 20, 30, and 40 °C) were varied to assess their impact on yield, antioxidant activity, mineral composition, cytotoxicity, and potential anticancer activity in Chinese hamster ovary (CHO) and hepatocyte-derived carcinoma (Huh-7) cell lines. The highest yield (51.34 ± 2.21 %) was obtained at 10 °C with 10 min of sonication, while the highest antioxidant activity (1.09 ± 0.12 mg g−1) was observed at 20 °C with 15 min of sonication. Mineral analysis showed that potassium was 64707.00 ± 53597.41 mg kg−1 at 10°C, 0-minute, and iron at 144.86 ± 48.06 mg kg−1 at 20°C, 0-minute. NMR analysis revealed the highest galactose content (46.16 ± 7.63 %) in the yield obtained at 20 °C with 0 min of sonication. FT-IR confirmed that temperature and sonication affected the rheological properties of agar. Biological assays showed anti-proliferative activity of 37.99 ± 8.70 % on Huh-7 and 38.59 ± 6.10 % on CHO cell lines (20°C, 0-minute), lowest wound healing 21.42 % and 26.49 % after 6 and 12 h (20°C, 0-minute). This is the first study that optimized temperature sonication enhances the yield and bioactivity of Gracilaria corticata salts, offering a scalable route to nutraceutical and pharmaceutical applications.