大黄鱼（Larimichthys crocea） Viperin的分子和功能特征。

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-06-13 DOI:10.1016/j.fsi.2025.110498

Wen Luo, Hongling Wu, Ying Huang, Kun Ye, Wenzheng Zou, Fang Han

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引用次数: 0

摘要

Viperin （virus inhibitory protein, endoplasmic reticum -associated, interferon-inducible）又称RSAD2 (radical SAM domain-containing 2)，是干扰素刺激基因之一，在细胞抗病毒应答中起重要作用。本研究通过研究大黄鱼（Larimichthys crocea） viperin基因的组织表达模式和对病毒感染的转录反应，以及通过RNA-seq转录组分析LcViperin在LCM10细胞中过表达时的差异表达基因（DEGs），对其进行鉴定和鉴定。结果表明，LcViperin在血液和免疫系统脏器（如脾、肾）中高表达，且LcViperin蛋白定位于内质网。大黄鱼虹膜病毒（LYCIV）感染和poly （I: C）模拟病毒感染可上调LcViperin的转录本水平。deg的GO和KEGG分析表明，LcViperin的过表达激活了巨噬细胞内吞途径和其他途径来消除病毒颗粒。流式细胞术分析显示，暴露于LcViperin蛋白后，LCM10细胞巨噬细胞吞噬活性增加，并诱导呼吸爆发。转录组分析结果将有助于了解宿主免疫反应和鱼Viperin蛋白对病原体刺激的潜在机制。

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Molecular and functional characterization of Viperin in large yellow croaker (Larimichthys crocea)

Viperin (virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible), also known as RSAD2 (radical SAM domain-containing 2), is one of the interferon-stimulated genes and plays an important role in cellular antiviral response. In this study, the viperin gene in large yellow croaker (Larimichthys crocea), designated LcViperin, was identified and characterized by investigating its tissue expression pattern and transcriptional response to viral infection and analyzing the differentially expressed genes (DEGs) upon overexpression of LcViperin in LCM10 cells revealed by RNA-seq transcriptome profiling. The results showed that LcViperin was highly expressed in the blood and organs of the immune system such as spleen and kidney, and the LcViperin protein was localized to the endoplasmic reticulum. The transcript level of LcViperin can be upregulated by large yellow croaker iridovirus (LYCIV) infection and the simulated viral infection of poly (I: C). GO and KEGG analysis of the DEGs demonstrated that the overexpression of LcViperin activated macrophage endocytosis pathway and other pathways to eliminate the viral particles. Flow cytometry analysis indicated an increase in macrophage phagocytotic activity and an induced respiratory burst in LCM10 cells upon exposure to LcViperin protein. The transcriptome analysis results would help to understand the host immune response and the potential mechanism of fish Viperin protein in response to pathogen stimulation.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.