生化分析浊度法的探索与应用进展

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-06-05 DOI:10.1016/j.trac.2025.118333

Jikai Wang , Ziyi Mo , Haitao Xie , Weiguo Wang , Suisui He , Jun Wang

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引用次数: 0

摘要

浊度测定法因其简单、快速、成本效益和高灵敏度而备受推崇，是定量浊度分析的基石。这些分析已广泛应用于各个领域，如水质评估，药物分析和生物调查。新试剂的出现，仪器的改进，以及精细的数据处理技术，大大拓宽了浊度法的应用范围。本文系统回顾了浊度法在定量生物标志物（如血液和尿液蛋白、酶）、微生物负荷、药物功效、生物分子相互作用和离子/分子浓度等方面的应用。同时，它批判性地评估了当前的局限性，并概述了准备重新定义浊度法的技术和方法前沿的新兴趋势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Advancements in the exploration and application of turbidimetric assays for biochemical analysis

Turbidimetric assays, esteemed for their simplicity, rapidity, cost-effectiveness, and high sensitivity, constitute a cornerstone of quantitative turbidity analysis. These assays have been extensively used in various fields, such as water quality assessments, pharmaceutical analyses, and biological investigations. The advent of novel reagents, instrument enhancements, and refined data processing techniques, has considerably broadened the application scope of turbidimetry. This manuscript provides a systematic review of turbidimetry's applications in quantifying biomarkers (e.g., blood and urinary proteins, enzymes), microbial loads, drug efficacy, biomolecular interactions, and ionic/molecular concentrations. Concurrently, it critically evaluates current limitations and outlines emerging trends poised to redefine the technological and methodological frontiers of turbidimetry.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.