VWA7 -一种推定的人类磷脂酰胆碱特异性磷脂酶CA。

IF 4.5 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Molecular Biology Pub Date : 2025-05-29 DOI:10.1016/j.jmb.2025.169239

Alexander Klipp, Christina Greitens, Jean-Christophe Leroux, Michael Burger

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引用次数: 0

摘要

四十多年来，哺乳动物磷脂酰胆碱特异性磷脂酶C （pc-PLC）的存在已被报道。然而，编码这种酶的基因至今还没有得到明确的确定。本研究提出了人类血管性血液病因子A结构域蛋白7 （VWA7）作为假定的pc-PLC，并对其潜在功能提供了一些生化见解。通过与蜡样芽孢杆菌pc-PLC的结构比较，发现VWA7的n端区域具有高度的相似性，并且具有保守的活性位点结构。研究了不同版本的VWA7在哺乳动物细胞中的定位和活性。最终，细菌中产生的VWA7变异体证实了pc-PLC的活性，最活跃的变异体达到733 mU/mg。这些发现为未来研究人类pc-PLC的作用以及研究较少的人类VWA7奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

VWA7 – A Putative Human Phosphatidylcholine-specific Phospholipase C

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VWA7 – A Putative Human Phosphatidylcholine-specific Phospholipase C

For over four decades, the existence of mammalian phosphatidylcholine-specific phospholipase C (pc-PLC) has been reported. However, the gene encoding this enzyme has not been unambiguously determined to date. This study presents human von Willebrand factor A domain-containing protein 7 (VWA7) as putative pc-PLC and provides some biochemical insights into its potential function. Structural comparison of the modelled N-terminal region of VWA7 with Bacillus cereus pc-PLC revealed high similarity and, remarkably, a conserved active site architecture. The localization and activity of different VWA7 versions were investigated in mammalian cells. Eventually, pc-PLC activity was confirmed for VWA7 variants produced in bacteria, with the most active variant reaching 733 mU/mg. These findings establish a basis for future investigations on the role of human pc-PLC as well as on the poorly studied human VWA7.

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来源期刊

Journal of Molecular Biology 生物-生化与分子生物学

CiteScore

11.30

自引率

1.80%

发文量

412

审稿时长

28 days

期刊介绍： Journal of Molecular Biology (JMB) provides high quality, comprehensive and broad coverage in all areas of molecular biology. The journal publishes original scientific research papers that provide mechanistic and functional insights and report a significant advance to the field. The journal encourages the submission of multidisciplinary studies that use complementary experimental and computational approaches to address challenging biological questions. Research areas include but are not limited to: Biomolecular interactions, signaling networks, systems biology; Cell cycle, cell growth, cell differentiation; Cell death, autophagy; Cell signaling and regulation; Chemical biology; Computational biology, in combination with experimental studies; DNA replication, repair, and recombination; Development, regenerative biology, mechanistic and functional studies of stem cells; Epigenetics, chromatin structure and function; Gene expression; Membrane processes, cell surface proteins and cell-cell interactions; Methodological advances, both experimental and theoretical, including databases; Microbiology, virology, and interactions with the host or environment; Microbiota mechanistic and functional studies; Nuclear organization; Post-translational modifications, proteomics; Processing and function of biologically important macromolecules and complexes; Molecular basis of disease; RNA processing, structure and functions of non-coding RNAs, transcription; Sorting, spatiotemporal organization, trafficking; Structural biology; Synthetic biology; Translation, protein folding, chaperones, protein degradation and quality control.