用热诱导抗体剥离法对黑质路易体α -突触核蛋白进行多重免疫荧光分析，揭示了其复杂的多层结构。

IF 3.4 2区医学 Q1 CLINICAL NEUROLOGY

Neuropathology and Applied Neurobiology Pub Date : 2025-06-01 DOI:10.1111/nan.70024

Dominik Hrabos, Satomi Hasegawa, Dorota Konickova

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引用次数: 0

摘要

在这项研究中，我们采用了一种经济实惠，节省组织，精确的同时多重免疫荧光方法，热诱导抗体剥离来鉴定和结构分析多巴胺能神经元中的黑质路易小体。对不同α -突触核蛋白表位和蛋白形态的分析揭示了路易小体几乎一致的洋葱样形态。n端和c端结构域主要可与抗体结合在机体外周外壳中。

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Multiplex Immunofluorescent Analysis of Alpha-Synuclein in Nigral Lewy Bodies With Heat-Induced Antibody Stripping Reveals an Intricate Multilayered Structure.

In the study, we employ an affordable, tissue-saving, and precise simultaneous multiplex immunofluorescence method with heat-induced antibody stripping to identify and structurally analyse nigral Lewy bodies in dopaminergic neurones. Analysis of different alpha-synuclein epitopes and proteoforms reveals an almost uniform, onion-like morphology of the Lewy bodies. The N-terminal and C-terminal domains are predominantly accessible to antibody binding in the peripheral shell of the bodies.

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来源期刊

Neuropathology and Applied Neurobiology 医学-病理学

CiteScore

8.20

自引率

2.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Neuropathology and Applied Neurobiology is an international journal for the publication of original papers, both clinical and experimental, on problems and pathological processes in neuropathology and muscle disease. Established in 1974, this reputable and well respected journal is an international journal sponsored by the British Neuropathological Society, one of the world leading societies for Neuropathology, pioneering research and scientific endeavour with a global membership base. Additionally members of the British Neuropathological Society get 50% off the cost of print colour on acceptance of their article.