betaproteobacter属Thauera和Aromatoleum的遗传操作。

4区生物学 Q3 Biochemistry, Genetics and Molecular Biology

Methods in enzymology Pub Date : 2025-01-01 Epub Date: 2025-01-28 DOI:10.1016/bs.mie.2025.01.009

Dominik Hege, Yvonne Gemmecker, Lina Clermont, Ivana Aleksic, Gabriela Oleksy, Maciej Szaleniec, Johann Heider

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引用次数: 0

摘要

大多数重组蛋白在大肠杆菌等模式宿主生物中表达。同时，大量的酶需要复杂的激活或特殊的辅因子，这是标准宿主无法提供的。betaproteobacteria Thauera和Aromatoleum可以按照本章所述的程序进入其中一些酶。所描述的方法能够将载体转化和偶联到这些物种中，作为替代的基因表达系统，从而允许对复杂重组蛋白及其生物技术应用进行基础研究。

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Genetic manipulation of the betaproteobacterial genera Thauera and Aromatoleum.

Most recombinant proteins are expressed in model host organisms like Escherichia coli. Meanwhile, a significant number of enzymes require complex activation or special cofactors not available from standard hosts. The betaproteobacteria Thauera and Aromatoleum allow access to some of these enzymes, following procedures described in this chapter. The methods described enable transformation and conjugation of vectors into these species as alternate gene expression systems which allow fundamental studies of complex recombinant proteins as well as their biotechnological application.

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来源期刊

Methods in enzymology 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

308

审稿时长

3-6 weeks

期刊介绍： The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.