{"title":"叶绿素荧光镜是一种用于教学和演示的观察叶绿素荧光在体内发射的装置。","authors":"João Serôdio","doi":"10.1007/s11120-025-01150-9","DOIUrl":null,"url":null,"abstract":"<p><p>In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.</p>","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":"163 3","pages":"26"},"PeriodicalIF":2.9000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000186/pdf/","citationCount":"0","resultStr":"{\"title\":\"The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.\",\"authors\":\"João Serôdio\",\"doi\":\"10.1007/s11120-025-01150-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.</p>\",\"PeriodicalId\":20130,\"journal\":{\"name\":\"Photosynthesis Research\",\"volume\":\"163 3\",\"pages\":\"26\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-04-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000186/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photosynthesis Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s11120-025-01150-9\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photosynthesis Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s11120-025-01150-9","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.
In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.
期刊介绍:
Photosynthesis Research is an international journal open to papers of merit dealing with both basic and applied aspects of photosynthesis. It covers all aspects of photosynthesis research, including, but not limited to, light absorption and emission, excitation energy transfer, primary photochemistry, model systems, membrane components, protein complexes, electron transport, photophosphorylation, carbon assimilation, regulatory phenomena, molecular biology, environmental and ecological aspects, photorespiration, and bacterial and algal photosynthesis.