叶绿素荧光镜是一种用于教学和演示的观察叶绿素荧光在体内发射的装置。

IF 2.9 3区 生物学 Q2 PLANT SCIENCES
João Serôdio
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引用次数: 0

摘要

体内叶绿素荧光长期以来一直被认为与光合作用密切相关,是广泛和复杂仪器的基础。虽然叶绿素荧光很容易在植物提取物中观察到,也可以通过表观荧光或共聚焦显微镜观察到,但在宏观样品中很少观察到叶绿素荧光,例如大型藻类的植物叶片。这项工作提出了一种“叶绿素荧光镜”,这种装置可以直接观察大量样品在体内发出的荧光。叶绿素荧光显微镜易于构建和操作,包括廉价的3d打印部件和现成的组件。它主要用于教学和科学演示活动,同时有可能引起那些意识到叶绿素荧光但通常从未观察到这种现象的人的兴趣。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The chlorophyll fluoroscope, a device to observe the in vivo emission of chlorophyll fluorescence for teaching and demonstration purposes.

In vivo chlorophyll fluorescence has long been known to be intimately related to photosynthesis, being the basis of widespread and sophisticated instrumentation. Although easily observed in plant extracts or using epifluorescence or confocal microscopes, chlorophyll fluorescence is seldom observed in macroscopic samples, such as plant leaves of macroalgae thalli. This work presents a 'chlorophyll fluoroscope', a device that allows the direct observation of the fluorescence emitted in vivo by large samples. The chlorophyll fluoroscope is easy to construct and operate, comprising inexpensive 3D-printed parts and off-the-shelve components. It is primarily intended for use in teaching and science demonstration events while having the potential to interest those who are aware of chlorophyll fluorescence yet often have never observed the phenomenon.

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来源期刊
Photosynthesis Research
Photosynthesis Research 生物-植物科学
CiteScore
6.90
自引率
8.10%
发文量
91
审稿时长
4.5 months
期刊介绍: Photosynthesis Research is an international journal open to papers of merit dealing with both basic and applied aspects of photosynthesis. It covers all aspects of photosynthesis research, including, but not limited to, light absorption and emission, excitation energy transfer, primary photochemistry, model systems, membrane components, protein complexes, electron transport, photophosphorylation, carbon assimilation, regulatory phenomena, molecular biology, environmental and ecological aspects, photorespiration, and bacterial and algal photosynthesis.
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