胆汁酸和牛磺胆酸介导的ahpnd致副溶血性弧菌毒力独立于LuxO-OpaR群体感应级联

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-04-21 DOI:10.1016/j.aquaculture.2025.742604

Ramya Kumar , Shin-Jen Lin , Chien-Kang Huang , Shih-Shun Lin , Hao-Ching Wang , Fernand F. Fagutao , Han-Ching Wang

{"title":"胆汁酸和牛磺胆酸介导的ahpnd致副溶血性弧菌毒力独立于LuxO-OpaR群体感应级联","authors":"Ramya Kumar , Shin-Jen Lin , Chien-Kang Huang , Shih-Shun Lin , Hao-Ching Wang , Fernand F. Fagutao , Han-Ching Wang","doi":"10.1016/j.aquaculture.2025.742604","DOIUrl":null,"url":null,"abstract":"<div><div>Acute hepatopancreatic necrosis disease (AHPND), a prevalent shrimp disease in Southeast Asia, is caused by a specific strain of Vibrio parahaemolyticus (VP) carrying a virulent pVA1 plasmid with PirABvp toxin genes. Several host and pathogenic factors affect AHPND pathogenesis. However, quorum sensing (QS), a cell density-based bacterial cell-to-cell communication that regulates virulence factors in Vibrios, mediated mechanisms of AHPND-causing VP are yet to be fully elucidated. Here, we used wild type V. parahaemolyticus, AHPND-causing strain 3HP, along with the 3HP LuxO and/or OpaR QS mutants (ΔopaRΔluxO, ΔopaR, ΔluxO, and luxOD47E) to investigate the effects of bile acids and taurocholate, dietary supplements given to shrimp to improve growth, on the pathogenicity of AHPND-causing VP. Our results suggest that biofilm formation and PirABvp toxin release in AHPND-causing wild type and QS mutant<del>s</del> VP strains were induced by bile acids and taurocholate. Furthermore, these dietary supplements affected genes related to flagellar apparatus (MotY, FlhB, FliK, flagellin), bacterial pilus (PilW), biofilm formation (VpsN), bile resistance (OmpT), quorum sensing (LuxR4425, LuxR2865, OpaR, LuxO) and PirABvp toxins (PirA, PirB). This suggests that bile acids or taurocholate may affect virulence mechanism of AHPND-causing VP in a LuxO-OpaR QS-independent manner. This study provided insights into host-pathogen interactions and molecular mechanisms of QS in biofilm formation and PirABvp toxin production in AHPND-causing VP.</div></div>","PeriodicalId":8375,"journal":{"name":"Aquaculture","volume":"606 ","pages":"Article 742604"},"PeriodicalIF":3.9000,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bile acid- and taurocholate-mediated virulence in AHPND-causing Vibrio parahaemolyticus is independent of the LuxO-OpaR quorum sensing cascade\",\"authors\":\"Ramya Kumar , Shin-Jen Lin , Chien-Kang Huang , Shih-Shun Lin , Hao-Ching Wang , Fernand F. Fagutao , Han-Ching Wang\",\"doi\":\"10.1016/j.aquaculture.2025.742604\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Acute hepatopancreatic necrosis disease (AHPND), a prevalent shrimp disease in Southeast Asia, is caused by a specific strain of Vibrio parahaemolyticus (VP) carrying a virulent pVA1 plasmid with PirABvp toxin genes. Several host and pathogenic factors affect AHPND pathogenesis. However, quorum sensing (QS), a cell density-based bacterial cell-to-cell communication that regulates virulence factors in Vibrios, mediated mechanisms of AHPND-causing VP are yet to be fully elucidated. Here, we used wild type V. parahaemolyticus, AHPND-causing strain 3HP, along with the 3HP LuxO and/or OpaR QS mutants (ΔopaRΔluxO, ΔopaR, ΔluxO, and luxOD47E) to investigate the effects of bile acids and taurocholate, dietary supplements given to shrimp to improve growth, on the pathogenicity of AHPND-causing VP. Our results suggest that biofilm formation and PirABvp toxin release in AHPND-causing wild type and QS mutant<del>s</del> VP strains were induced by bile acids and taurocholate. Furthermore, these dietary supplements affected genes related to flagellar apparatus (MotY, FlhB, FliK, flagellin), bacterial pilus (PilW), biofilm formation (VpsN), bile resistance (OmpT), quorum sensing (LuxR4425, LuxR2865, OpaR, LuxO) and PirABvp toxins (PirA, PirB). This suggests that bile acids or taurocholate may affect virulence mechanism of AHPND-causing VP in a LuxO-OpaR QS-independent manner. This study provided insights into host-pathogen interactions and molecular mechanisms of QS in biofilm formation and PirABvp toxin production in AHPND-causing VP.</div></div>\",\"PeriodicalId\":8375,\"journal\":{\"name\":\"Aquaculture\",\"volume\":\"606 \",\"pages\":\"Article 742604\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-04-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquaculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0044848625004909\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044848625004909","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

摘要

急性肝胰腺坏死病（AHPND）是一种在东南亚流行的虾类疾病，由一种携带piabvp毒素基因的毒性pVA1质粒的副溶血性弧菌（VP）引起。多种宿主和致病因素影响AHPND的发病机制。然而，群体感应（quorum sensing， QS）是一种基于细胞密度的细菌间通讯，可调节弧菌的毒力因子，其介导的ahpnd引起VP的机制尚未完全阐明。在这里，我们使用野生型副溶血性弧菌，引起ahpnd的菌株3HP，以及3HP LuxO和/或OpaR QS突变体（ΔopaRΔluxO， ΔopaR， ΔluxO和luxOD47E）来研究胆汁酸和牛牛胆酸对ahpnd引起的VP致病性的影响。结果表明，胆汁酸和牛磺酸胆酸可诱导ahpnd野生型和QS突变型VP菌株形成生物膜并释放PirABvp毒素。此外，这些膳食补充剂影响了鞭毛器官（MotY、FlhB、FliK、鞭毛蛋白）、细菌菌毛（PilW）、生物膜形成（VpsN）、胆汁抗性（OmpT）、群体感应（LuxR4425、LuxR2865、OpaR、LuxO）和PirABvp毒素（PirA、PirB）相关基因。这表明胆汁酸或牛磺胆酸可能以不依赖于LuxO-OpaR qs的方式影响ahpnd致VP的毒力机制。本研究揭示了致病菌与宿主的相互作用以及QS在ahpnd致VP生物膜形成和PirABvp毒素产生中的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Bile acid- and taurocholate-mediated virulence in AHPND-causing Vibrio parahaemolyticus is independent of the LuxO-OpaR quorum sensing cascade

Acute hepatopancreatic necrosis disease (AHPND), a prevalent shrimp disease in Southeast Asia, is caused by a specific strain of Vibrio parahaemolyticus (VP) carrying a virulent pVA1 plasmid with PirAB^vp toxin genes. Several host and pathogenic factors affect AHPND pathogenesis. However, quorum sensing (QS), a cell density-based bacterial cell-to-cell communication that regulates virulence factors in Vibrios, mediated mechanisms of AHPND-causing VP are yet to be fully elucidated. Here, we used wild type V. parahaemolyticus, AHPND-causing strain 3HP, along with the 3HP LuxO and/or OpaR QS mutants (ΔopaRΔluxO, ΔopaR, ΔluxO, and luxO^D47E) to investigate the effects of bile acids and taurocholate, dietary supplements given to shrimp to improve growth, on the pathogenicity of AHPND-causing VP. Our results suggest that biofilm formation and PirAB^vp toxin release in AHPND-causing wild type and QS mutants VP strains were induced by bile acids and taurocholate. Furthermore, these dietary supplements affected genes related to flagellar apparatus (MotY, FlhB, FliK, flagellin), bacterial pilus (PilW), biofilm formation (VpsN), bile resistance (OmpT), quorum sensing (LuxR4425, LuxR2865, OpaR, LuxO) and PirAB^vp toxins (PirA, PirB). This suggests that bile acids or taurocholate may affect virulence mechanism of AHPND-causing VP in a LuxO-OpaR QS-independent manner. This study provided insights into host-pathogen interactions and molecular mechanisms of QS in biofilm formation and PirAB^vp toxin production in AHPND-causing VP.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.