用于生物和环境样品中HSO3−敏感检测的选择性荧光探针的开发

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-17 DOI:10.1016/j.saa.2025.126242

He Gao , Xianjing Zhang , Haiyan Ma , Yuyao Li , Dongyuan Xu , Longbin Xu

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引用次数: 0

摘要

二氧化硫（SO2）是一种重要的大气污染物和食品添加剂，过量食用亚硫酸盐（HSO3−）与呼吸系统和心血管疾病等健康风险有关。可靠、灵敏的HSO3 -检测对于食品安全和环境监测至关重要。在这里，我们提出了一种新的荧光探针（HMQ），用于选择性检测生物和环境样品中的HSO3−。由于亲核加成机制，探针表现出明显的“ON-OFF”荧光响应，使HSO3−比其他阴离子具有高选择性。HMQ已成功应用于活细胞外源HSO3−检测和食品样品亚硫酸盐污染监测。此外，浸渍HMQ的试纸条提供了一种简单快速的方法来检测水溶液和气体环境中的HSO3−(SO2)。该探针在食品和血清样品中均表现出良好的灵敏度，具有线性荧光响应和高回收率。这项工作为亚硫酸盐检测提供了一种多功能工具，在食品安全、环境监测和疾病研究中具有潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Development of a selective fluorescent probe for sensitive detection of HSO3− in biological and environmental samples

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Development of a selective fluorescent probe for sensitive detection of HSO3− in biological and environmental samples

Sulfur dioxide (SO₂) is a significant atmospheric pollutant and food additive, with excessive sulfite (HSO₃⁻) consumption linked to health risks such as respiratory and cardiovascular disorders. Reliable, sensitive detection of HSO₃⁻ is essential for food safety and environmental monitoring. Here, we present a novel fluorescent probe (HMQ) for the selective detection of HSO₃⁻ in biological and environmental samples. The probe exhibits a distinct “ON-OFF” fluorescence response due to a nucleophilic addition mechanism, enabling high selectivity for HSO₃⁻ over other anions. HMQ was successfully applied for detecting exogenous HSO₃⁻ in living cells and for monitoring sulfite contamination in food samples. Additionally, test strips impregnated with HMQ provide a simple and rapid method for detecting HSO₃⁻ (SO₂) in aqueous solutions and gaseous environments. The probe demonstrates excellent sensitivity, with linear fluorescence responses and high recovery rates in both food and serum samples. This work provides a versatile tool for sulfite detection, with potential applications in food safety, environmental monitoring, and disease research.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.