rna脱帽蛋白GhLSM1BS的鉴定和过表达：通过上调油菜素内酯生物合成促进棉花体细胞胚胎发生

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2025-04-15 DOI:10.1111/pbi.70090

Zheng Yang, Jie He, Shuqian Yao, Xuxin Li, Chang Du, Juwu Gong, Haoliang Yan, Yanpeng Zhao, Yang Li, Youlu Yuan, Haihong Shang

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引用次数: 0

摘要

我们发现了 GhLSM1B 的两种剪接变体（GhLSM1BS 和 GhLSM1BL），它们具有不同的表达模式和预测的三维结构，尽管具有相同的核定位。在体细胞胚胎发生过程中，过表达 GhLSM1BS（而非 GhLSM1BL）可加速棉花胼胝体的增殖并改变细胞形态，同时还可改变 CYP450 家族基因的表达和提高黄铜类固醇的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Identification and overexpression of RNA-decapping protein GhLSM1BS: Enhancing cotton somatic embryogenesis through up-regulating brassinosteroid biosynthesis

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Identification and overexpression of RNA-decapping protein GhLSM1BS: Enhancing cotton somatic embryogenesis through up-regulating brassinosteroid biosynthesis

We identified two splicing variants of GhLSM1B (GhLSM1BS and GhLSM1BL) with distinct expression patterns and predicted 3D structures, despite sharing the same nuclear localization. Overexpression of GhLSM1BS, but not GhLSM1BL, accelerated cotton callus proliferation and altered cell morphology during somatic embryogenesis, accompanied by altered expression of CYP450 family genes and elevated brassinosteroid levels.

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.