荧光生物传感器定量植物生物学。

IF 21.3 1区 生物学 Q1 PLANT SCIENCES
James H Rowe, Max Josse, Bijun Tang, Alexander M Jones
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引用次数: 0

摘要

植物生物学正在经历一场空间组学革命,但这些方法仅限于植物状态的快照。直接的、遗传编码的荧光生物传感器补充了组学方法,为研究人员提供了从快速亚细胞动力学到活植物的有机模式,在多个尺度上评估能量、代谢和信号分子的工具。这篇综述着重介绍了生物传感器如何在这些尺度上照亮植物生物学,以及它们所贡献的主要发现。我们还讨论了影响生物传感器工程、部署、成像和分析的核心原则和常见陷阱,以帮助有抱负的生物传感器研究人员。创新技术正在推动生物和技术的发展,与其他方法协同生物传感器研究并扩大体内定量生物学的范围。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying Plant Biology with Fluorescent Biosensors.

Plant biology is undergoing a spatial omics revolution, but these approaches are limited to snapshots of a plant's state. Direct, genetically encoded fluorescent biosensors complement the omics approaches, giving researchers tools to assess energetic, metabolic, and signaling molecules at multiple scales, from fast subcellular dynamics to organismal patterns in living plants. This review focuses on how biosensors illuminate plant biology across these scales and the major discoveries to which they have contributed. We also discuss the core principles and common pitfalls affecting biosensor engineering, deployment, imaging, and analysis to help aspiring biosensor researchers. Innovative technologies are driving forward developments both biological and technical with implications for synergizing biosensor research with other approaches and expanding the scope of in vivo quantitative biology.

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来源期刊
Annual review of plant biology
Annual review of plant biology 生物-植物科学
CiteScore
40.40
自引率
0.40%
发文量
29
期刊介绍: The Annual Review of Plant Biology is a peer-reviewed scientific journal published by Annual Reviews. It has been in publication since 1950 and covers significant developments in the field of plant biology, including biochemistry and biosynthesis, genetics, genomics and molecular biology, cell differentiation, tissue, organ and whole plant events, acclimation and adaptation, and methods and model organisms. The current volume of this journal has been converted from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.
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