玉米芯粉诱导尿毒枝孢杆菌HSK8产生afb1降解酶解毒

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-03-25 DOI:10.1016/j.toxicon.2025.108333

Mengxiong Xiang , Ernuo Tian , Shuting Wang , Siyao Wang , Runmei Zhang , Jianyi Guo , Ming Liu , Qiang Ding , Jun Cai

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引用次数: 0

摘要

黄曲霉毒素B1 （AFB1）是一种高毒性真菌毒素，污染食物来源并造成严重的健康风险。本研究检测了尿嘧啶枝孢杆菌HSK8降解AFB1的能力。结果表明，玉米芯粉经过高温处理后，水解产生糠醛和香兰素等衍生物，刺激C. uredinicola HSK8产生多种afb1降解酶。在玉米芯粉含量为3%的发酵液中，总酶活性达到3750 U/mL。进一步分离纯化了两种afb1降解酶，并对其进行了分析。结果表明，酶能同时破坏呋喃环和苯环，表现出较强的催化活性。结果表明，玉米芯粉作为复合碳源不仅能促进红孢杆菌的生长，还能诱导afb1降解酶的产生。因此，玉米芯粉培养的C. uredinicola HSK8发酵液对食品和饲料中AFB1的解毒具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Using corn cob powder to induce AFB1-degrading enzyme production in Cladosporium uredinicola HSK8 for detoxification purposes

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Using corn cob powder to induce AFB1-degrading enzyme production in Cladosporium uredinicola HSK8 for detoxification purposes

Aflatoxin B₁ (AFB₁) is a highly toxic mycotoxin that contaminates food sources and poses serious health risks. This study examines Cladosporium uredinicola HSK8's ability to degrade AFB₁. It was demonstrated that the hydrolysis of corn cob powder, following high-temperature treatment, generates derivatives such as furfural and vanillin, which stimulate the production of various AFB₁-degrading enzymes by C. uredinicola HSK8. The total enzymatic activity reached a peak of 3750 U/mL in fermentation broth with 3 % corn cob powder. Two AFB₁-degrading enzymes were further isolated, purified and analyzed. The results showed that enzymes can disrupt both the furan and benzene rings, exhibiting strong catalytic activity. The results suggested corn cob powder as a complex carbon source not only promotes the growth of C. uredinicola but also induces the production of AFB₁-degrading enzymes. Therefore, the fermentation broth of C. uredinicola HSK8 cultured with corn cob powder holds great potential for the detoxification of AFB₁ in food and feed.

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.