DNA与硼簇金属配合物复合材料的合成及其组装成具有基因沉默活性的功能纳米颗粒

IF 2.6 4区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

ChemNanoMat Pub Date : 2025-01-24 DOI:10.1002/cnma.202400606

Krzysztof Śmiałkowski, Katarzyna Bednarska-Szczepaniak, Katarzyna Kulik, Justyna Suwara, Bohumir Grűner, Aleksander Foryś, Barbara Nawrot, Zbigniew J. Leśnikowski

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引用次数: 0

摘要

在此，我们描述了一种基于硼簇金属复合物（金属碳硼烷）和治疗性核酸的复合材料的新型纳米构建块，并将它们组装成具有EGFR癌基因沉默活性的功能纳米颗粒。在这个方向上，开发了一种金属碳硼烷，3,3 ' -钴-双（1,2-二碳内酯）（−1）离子的低官能化方法，以及一种自动合成金属碳硼烷核上dna寡核苷酸的方法。获得的构建块形成具有“菊花样”拓扑结构的纳米颗粒，在体外胰腺癌细胞系PANC-1中有效地携带缓慢释放的反义抗EGFR寡核苷酸沉默EGFR基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Synthesis of Composites of DNA and Boron Cluster-Metal Complexes and their Assembly into Functional Nanoparticles with Gene Silencing Activity

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Synthesis of Composites of DNA and Boron Cluster-Metal Complexes and their Assembly into Functional Nanoparticles with Gene Silencing Activity

Herein, we describe a new type of building block for nanoconstruction based on the composite of a boron cluster metal-complex (metallacarborane) and therapeutic nucleic acid and their assembly into functional nanoparticles with EGFR oncogene silencing activity. In this direction, a method for oligofunctionalization of a metallacarborane, 3,3’-cobalt-bis(1,2-dicarbollide)(−1) ion, and an automated method for synthesis of DNA-oligonucleotides attached to the metallacarborane core is developed. The obtained building blocks forms nanoparticles with a “chrysanthemum-like” topology that carry a slowly releasing antisense anti-EGFR oligonucleotide silencing EGFR gene efficiently in vitro in a pancreatic carcinoma cell line PANC-1.

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来源期刊

ChemNanoMat Energy-Energy Engineering and Power Technology

CiteScore

6.10

自引率

2.60%

发文量

236

期刊介绍： ChemNanoMat is a new journal published in close cooperation with the teams of Angewandte Chemie and Advanced Materials, and is the new sister journal to Chemistry—An Asian Journal.