新半胱氨酸摇篮家族的一个决定性成员是 elegans 中传递皮肤损伤信号的 aECM 蛋白。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-03-20 eCollection Date: 2025-03-01 DOI:10.1371/journal.pgen.1011593
Thomas Sonntag, Shizue Omi, Antonina Andreeva, Claire Valotteau, Jeanne Eichelbrenner, Andrew D Chisholm, Jordan D Ward, Nathalie Pujol
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引用次数: 0

摘要

顶端细胞外基质(aecm)作为关键的屏障,与表皮沟通,触发损伤或感染后的保护反应。在秀丽隐杆线虫中,表皮aECM,即角质层,由表皮产生,表面有周期性的周沟。我们之前的研究表明,缺乏角质层沟槽的突变体表现出持续的免疫激活(PIA),为研究角质层损伤和免疫应答之间的联系提供了一个有价值的模型。在基因抑制筛选中,我们发现spia-1是沟状胶原下游和免疫信号上游的关键基因。Spia-1的表达在幼虫发育过程中振荡,在每次蜕皮期间达到峰值,并形成图案表皮成分。它对一种定位于犁沟的分泌蛋白进行编码。SPIA-1与其他aECM蛋白共享一个新的半胱氨酸摇篮结构域。SPIA-1介导对沟槽损失的免疫激活,并被认为是角质层损伤的细胞外信号激活剂。这项研究为秀丽隐杆线虫角质层完整性和表皮免疫激活之间复杂的相互作用提供了分子视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A defining member of the new cysteine-cradle family is an aECM protein signalling skin damage in C. elegans.

Apical extracellular matrices (aECMs) act as crucial barriers, and communicate with the epidermis to trigger protective responses following injury or infection. In Caenorhabditis elegans, the skin aECM, the cuticle, is produced by the epidermis and is decorated with periodic circumferential furrows. We previously showed that mutants lacking cuticle furrows exhibit persistent immune activation (PIA), providing a valuable model to study the link between cuticle damage and immune response. In a genetic suppressor screen, we identified spia-1 as a key gene downstream of furrow collagens and upstream of immune signalling. spia-1 expression oscillates during larval development, peaking between each moult together with patterning cuticular components. It encodes a secreted protein that localises to furrows. SPIA-1 shares a novel cysteine-cradle domain with other aECM proteins. SPIA-1 mediates immune activation in response to furrow loss and is proposed to act as an extracellular signal activator of cuticle damage. This research provides a molecular insight into intricate interplay between cuticle integrity and epidermal immune activation in C. elegans.

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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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