好氧氧化催化剂乙酰丙酮钒促进发光胺化学发光对碱性磷酸酶和抗坏血酸的敏感检测。

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-03-17 DOI:10.1007/s00216-025-05827-x

Wei Zhang, Yequan Chen, Kaixiang Ji, Zhiyong Dong, Abubakar Abdussalam, Yuriy T. Zholudov, Wenyue Gao, Yu Tian, Guobao Xu

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引用次数: 0

摘要

乙酰丙酮钒（VO(acac)2）是一种众所周知的环境友好型有氧氧化催化剂。首次在水介质中作为发光氨化学发光（CL）的高效助反应物被开发。鲁米诺- vo (acac)2体系产生强烈的CL，抗坏血酸对其CL有明显的抑制作用。基于抗坏血酸的强猝灭作用，将鲁米诺- vo (acac)2 CL用于定量临床重要的抗坏血酸和碱性磷酸酶（ALP），通过酶促l-抗坏血酸2-磷酸转化为抗坏血酸。检测抗坏血酸和碱性磷酸酶的线性范围分别为1 ~ 100 μM和0.005 ~ 200 U/L。抗坏血酸和碱性磷酸酶的检出限分别为0.5 μM和0.005 U/L。该方法对被测生物分子和金属离子的碱性磷酸酶具有较高的选择性。在人血清样品中也显示出良好的回收率。

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Aerobic oxidation catalyst vanadyl acetylacetonate boosts luminol chemiluminescence for sensitive detection of alkaline phosphatase and ascorbic acid

Vanadyl acetylacetonate, VO(acac)₂, is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac)₂ system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac)₂ CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.