耐碳青霉烯类铜绿假单胞菌亚胺培南-瑞巴坦药敏试验的评价：微孔板、圆盘扩散和MTS梯度条与肉汤微量稀释的比较。

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-03-13 DOI:10.1007/s10096-025-05058-z

Adrien Biguenet, Léa Devoos, Julie Rousselot, Maxime Bour, Damien Fournier, Katy Jeannot

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引用次数: 0

摘要

以微量肉汤稀释（BMD）为参比，对148株头孢他啶和耐亚胺培南铜绿假单胞菌的亚胺培南-瑞巴坦敏感性进行了评价。EUMDROXF®Sensititre微孔板的准确度最高（CA = 93.2%, EA = 93.9%，偏置差+ 21.8%）。而MTS梯度条表现出良好的性能(CA = 84.5%；EA = 89.9%，偏差差= 21.0%)，圆盘法错误分类25.8%（16/62）耐药菌株。鉴于这些结果，建议在铜绿假单胞菌菌株的亚胺培南-瑞巴坦检测中考虑圆盘扩散（20-24 mm）的技术不确定区域（ATU）。

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Evaluation of imipenem-relebactam susceptibility testing in carbapenem resistant Pseudomonas aeruginosa: comparison of sensititre microplates, disc diffusion, and MTS gradient strips with broth microdilution.

Four commercial tests EUMDROXF^® Sensititre microplates, MTS gradient strips, and disc diffusion were evaluated for imipenem-relebactam susceptibility in 148 ceftazidime and imipenem-resistant Pseudomonas aeruginosa strains, using broth microdilution (BMD) as the reference. EUMDROXF^® Sensititre microplates showed the highest accuracy (CA = 93.2%, EA = 93.9%, and bias difference + 21.8%). While the MTS gradient strips showed acceptable performance (CA = 84.5%; EA = 89.9%, and difference of bias = 21.0%), the disc method misclassified 25.8% (16/62) of the resistant strains. Given these results, considering an Area of Technical Uncertainty (ATU) for disc diffusion (20-24 mm) in imipenem-relebactam testing is recommended for P. aeruginosa strains.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.