新型MBT LipidART模块（Bruker Daltonics）检测大肠埃希菌和肺炎克雷伯菌粘菌素耐药性的性能评价

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

European Journal of Clinical Microbiology & Infectious Diseases Pub Date : 2025-03-12 DOI:10.1007/s10096-025-05099-4

Julija Germ, Mateja Pirs

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引用次数: 0

摘要

我们在MALDI Biotyper®Sirius系统（Bruker Daltonics）上通过分析负离子模式下的脂质a谱，评估了MBT LipidART模块在大肠杆菌（EC）和肺炎克雷伯菌（KPN）中快速检测粘菌素耐药性的能力。98.3%的EC （N = 58）和85.0%的KPN （N = 40）完全一致。挑战包括校准困难，兼容设备的有限可用性以及产生无效结果的黏液和粘附KPN分离物的问题。虽然MBT LipidART模块有望作为一种快速检测粘菌素耐药性的工具，但与KPN相比，其在EC中的表现明显更好。

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Performance evaluation of a novel MBT LipidART module (Bruker Daltonics) for detection of colistin resistance in Escherichia coli and Klebsiella pneumoniae.

We evaluated the MBT LipidART module on a MALDI Biotyper® Sirius System (Bruker Daltonics) for the rapid detection of colistin resistance in Escherichia coli (EC) and Klebsiella pneumoniae (KPN) by analysing lipid A profiles in negative ion mode. Categorical agreement was achieved for 98.3% EC (N = 58) and 85.0% KPN (N = 40). Challenges included calibration difficulties, limited availability of compatible equipment and issues with mucoid and adherent KPN isolates that yielded invalid results. While MBT LipidART module shows promise as a rapid tool for detection of colistin resistance, its performance was notably better for EC compared to KPN.

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来源期刊

European Journal of Clinical Microbiology & Infectious Diseases 医学-传染病学

CiteScore

10.40

自引率

2.20%

发文量

138

审稿时长

1 months

期刊介绍： EJCMID is an interdisciplinary journal devoted to the publication of communications on infectious diseases of bacterial, viral and parasitic origin.