EWS-RNA结合蛋白1:通过构象动力学研究了解尤文氏肉瘤的结构。

IF 2.3 4区 医学 Q3 ONCOLOGY
Saba Shahzadi, Muhammad Yasir, Andrzej Kloczkowski, Mubashir Hassan
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引用次数: 0

摘要

背景:先前的研究表明,蛋白质通过下游信号级联的相互作用,在包括尤文氏肉瘤在内的各种肉瘤的预后和治疗中发挥重要作用。然而,关于EWSR1的结构构象及其与RNA分子相互作用在Ews-ing肉瘤预后中的结构意义的了解有限。目的:正在进行的研究的主要目的是确定EWSR1如何参与尤文氏肉瘤。目的:探讨Ewing肉瘤中EWSR1结构的复杂性及其与RNA的构象相互作用。方法:采用比较建模的方法分别预测EWSR1结构域,并利用DEMO服务器将其组装成一个结构单元。此外,预测了与EWSR1相互作用的RNA基序,并利用RNAComposer建立了三维模型。通过蛋白- rna对接和MD模拟研究,检测对接的EWSR1-RNA复合物的分子间相互作用和稳定性行为。结果:总体结果揭示了EWSR1的结构及其与RNA的相互作用,这些相互作用可能在调控基因表达的共转录和转录后调控中发挥重要作用。结论:综上所述,我们的研究结果表明,EWSR1可能是诊断和治疗尤文氏肉瘤的一个有用的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EWS-RNA Binding Protein 1: Structural Insights into Ewing Sarcoma by Conformational Dynamics Investigations.

Background: Prior research has demonstrated that proteins play a significant role in the prognosis and treatments of various sarcomas, including Ewing sarcoma through the interplay of downstream signaling cascades. However, there is limited understanding about the strcucture conformation of EWSR1 and its structural implication in the prognosis of Ews-ing Sarcoma by interaction with RNA molecules.

Aims: The primary goal of ongoing research is to determine how EWSR1 contributes to Ewing sarcoma.

Objective: The current study explores the complexity of EWSR1 structure and its conforma-tional interactions with RNA in relation to Ewing sarcoma.

Methods: Here, we employed a comparative modeling approach to predict EWSR1 domains separately and assembled them into one structural unit using a DEMO server. Additionally, the RNA motifs interacting with EWSR1 were predicted, and the 3D model was built using RNAComposer. Protein-RNA docking and MD simulation studies were carried out to check the intermolecular interactions and stability behavior of docked EWSR1-RNA complexes.

Results: The overall results explore the structural insights into EWSR1 and their interactions with RNA, which may play a momentous role in co- and post-transcriptional regulation to control gene expression.

Conclusion: Taken togather, our findings suggest that EWSR1 may be a useful therapeutic target for the diagnosis and management of Ewing sarcoma.

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来源期刊
Current cancer drug targets
Current cancer drug targets 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
105
审稿时长
1 months
期刊介绍: Current Cancer Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular drug targets involved in cancer, e.g. disease specific proteins, receptors, enzymes and genes. Current Cancer Drug Targets publishes original research articles, letters, reviews / mini-reviews, drug clinical trial studies and guest edited thematic issues written by leaders in the field covering a range of current topics on drug targets involved in cancer. As the discovery, identification, characterization and validation of novel human drug targets for anti-cancer drug discovery continues to grow; this journal has become essential reading for all pharmaceutical scientists involved in drug discovery and development.
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