{"title":"从2023年和2024年爆发的分子分析揭示了印度小反刍动物害虫病毒谱系IV的独家传播","authors":"Asha Anand, Swathi Mahadevappa, Rakshit Ojha, Archana Pal, Shweta Priya, Annett Helcita Dsouza, Prajakta Prashant Bokade, Kirubakaran Vinod Kumar, Divakar Hemadri, Baldev Raj Gulati, Vinayagamurthy Balamurugan","doi":"10.1007/s00705-025-06254-0","DOIUrl":null,"url":null,"abstract":"<div><p>Peste des petits ruminants (PPR) is a highly contagious disease affecting sheep and goats, caused by PPR virus (PPRV), a member of the genus <i>Morbillivirus</i>. In 2023 and 2024, on testing of suspected clinical samples for PPR received through passive surveillance of sheep and goat flocks in various states of India resulted in the documentation of 15 laboratory-confirmed outbreaks of PPR. During the outbreak investigation, morbidity and significant mortality rates were observed, and clinical samples collected from the affected flocks were tested using ELISA and RT-PCR, confirming the presence of PPRV. We studied in detail nine out of 15 confirmed outbreaks for characterization of virus including partial sequencing of nucleocapsid (N) and fusion (F) genes of the PPRV. In addition, the virus from each outbreak was isolated from tissue and swab samples by blind passage in Vero cells. Phylogenetic analysis based on partial N gene sequences revealed that all of the Indian isolates belonged to Asian lineage IV. This study confirms that all of the cases of PPR reported so far in India have been caused by PPRV lineage IV.</p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 4","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular insights from 2023 and 2024 outbreaks reveal exclusive circulation of peste des petits ruminants virus lineage IV in India\",\"authors\":\"Asha Anand, Swathi Mahadevappa, Rakshit Ojha, Archana Pal, Shweta Priya, Annett Helcita Dsouza, Prajakta Prashant Bokade, Kirubakaran Vinod Kumar, Divakar Hemadri, Baldev Raj Gulati, Vinayagamurthy Balamurugan\",\"doi\":\"10.1007/s00705-025-06254-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Peste des petits ruminants (PPR) is a highly contagious disease affecting sheep and goats, caused by PPR virus (PPRV), a member of the genus <i>Morbillivirus</i>. In 2023 and 2024, on testing of suspected clinical samples for PPR received through passive surveillance of sheep and goat flocks in various states of India resulted in the documentation of 15 laboratory-confirmed outbreaks of PPR. During the outbreak investigation, morbidity and significant mortality rates were observed, and clinical samples collected from the affected flocks were tested using ELISA and RT-PCR, confirming the presence of PPRV. We studied in detail nine out of 15 confirmed outbreaks for characterization of virus including partial sequencing of nucleocapsid (N) and fusion (F) genes of the PPRV. In addition, the virus from each outbreak was isolated from tissue and swab samples by blind passage in Vero cells. Phylogenetic analysis based on partial N gene sequences revealed that all of the Indian isolates belonged to Asian lineage IV. This study confirms that all of the cases of PPR reported so far in India have been caused by PPRV lineage IV.</p></div>\",\"PeriodicalId\":8359,\"journal\":{\"name\":\"Archives of Virology\",\"volume\":\"170 4\",\"pages\":\"\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00705-025-06254-0\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06254-0","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
Molecular insights from 2023 and 2024 outbreaks reveal exclusive circulation of peste des petits ruminants virus lineage IV in India
Peste des petits ruminants (PPR) is a highly contagious disease affecting sheep and goats, caused by PPR virus (PPRV), a member of the genus Morbillivirus. In 2023 and 2024, on testing of suspected clinical samples for PPR received through passive surveillance of sheep and goat flocks in various states of India resulted in the documentation of 15 laboratory-confirmed outbreaks of PPR. During the outbreak investigation, morbidity and significant mortality rates were observed, and clinical samples collected from the affected flocks were tested using ELISA and RT-PCR, confirming the presence of PPRV. We studied in detail nine out of 15 confirmed outbreaks for characterization of virus including partial sequencing of nucleocapsid (N) and fusion (F) genes of the PPRV. In addition, the virus from each outbreak was isolated from tissue and swab samples by blind passage in Vero cells. Phylogenetic analysis based on partial N gene sequences revealed that all of the Indian isolates belonged to Asian lineage IV. This study confirms that all of the cases of PPR reported so far in India have been caused by PPRV lineage IV.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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