Lin Xin, Hong-Liang Xiao, Mei-Di Li, Yi-Kuan Ji, Wen-Zi Liu, Xiao-Ping Liao, Yang Yu, Dong-Hao Zhao
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In Vitro Plasma Protein Binding Determination of Lefamulin by Equilibrium Dialysis and High-Performance Liquid Chromatography Coupled With Evaporative Light Scattering Detector
A credible method utilizing equilibrium dialysis and high-performance liquid chromatography with an evaporative light scattering detector (HPLC-ELSD) was developed to determine the plasma protein binding of lefamulin in swine. The method demonstrated excellent linearity, with a logarithmic calibration curve in the range of 0.1 to 2 μg/mL (r > 0.999). Extraction recoveries from plasma samples ranged from 81.0% to 90.8%, with coefficients of variation less than 7.21%. The limits of detection and quantitation were established at 0.05 and 0.1 μg/mL, respectively. The mean plasma protein binding rates of lefamulin were found to be 70.2 ± 8.0%, 68.4 ± 4.9%, and 68.4 ± 3.6%, respectively, with coefficients of variation below 11.4%. This method was satisfactorily applied to the determination of plasma protein binding rate of lefamulin with high precision, accuracy, and sensitivity.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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