高m/z生物离子形成、传输、分析和检测的质谱仪的发展:个人观点。

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Iain D G Campuzano, Joseph A Loo
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引用次数: 0

摘要

质谱(MS)已经成为几乎所有学术、制药和生物制药分析实验室的基本工具。高m/z离子的形成、传输、分析和检测是质谱研究和应用的一个相对较新的领域,在过去的二十年里,人们对质谱的兴趣急剧增加。在这里,我们深入研究了质谱的这一令人兴奋的方面,讨论了质谱仪器是如何为原生质谱分析而改进和发展的。我们涵盖了早期开创性的实验,显示了高m/z离子的形成,传输,以及在气相中蛋白质结构的保存。此外,我们还讨论了具有先进的高m/z离子产生,传输,分析和检测的特定仪器优化和修改,为气相结构生物学的研究领域做出贡献。原生质谱样品引入方法,新兴技术和未来的观点也进行了检查。最后,我们分享个人观点、观察和经验,这些对社区来说是新的或以前未发表的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evolution of Mass Spectrometers for High m/z Biological Ion Formation, Transmission, Analysis and Detection: A Personal Perspective.

Mass spectrometry (MS) has become an essential tool in virtually all academic, pharmaceutical, and biopharmaceutical analytical laboratories. The specialized and bespoke area of MS research and application of high m/z ion (>m/z 6000 and high mass, >150 kDa) formation, transmission, analysis, and detection is a relatively new area of focus for MS that has seen dramatic acceleration in interest over the last two decades. Herein we delve into this exciting aspect of MS, discussing how MS instrumentation has been refined and evolved for native-MS analysis. We cover the early groundbreaking experiments showing high m/z ion formation, transmission, and preservation of protein structure in the gas phase. Additionally, we discuss specific instrument optimizations and modifications that have advanced high m/z ion generation, transmission, analysis, and detection, contributing to the research area known as gas-phase structural biology. Native-MS sample introduction methods, emerging technologies, and future perspectives are also examined. Finally, we share personal opinions, observations, and experiences that are new to the community or previously unpublished.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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