{"title":"Musae真菌黑素的鉴定及其抗氧化和光保护作用","authors":"Fatma Meryem Bozhuyuk, Murat Ozdal","doi":"10.1007/s00203-025-04279-2","DOIUrl":null,"url":null,"abstract":"<div><p>Melanin is a dark colored pigment produced by various living organisms. In this study, the melanin-producing fungus was isolated from a washing machine and identified as <i>Scolecobasidium musae</i> by 18 S rDNA analysis. Melanin production by <i>S. musae</i> was reported for the first time. Melanin was obtained from the dark-colored biomass produced by <i>S. musae</i> in submerged culture. The pigment was then purified, characterized, and identified. It was determined that purified melanin was dissolved easily in alkaline solutions but insoluble in organic solvents. Devices such as UV-Vis spectroscopy, XRD, FTIR, NMR, EPR, and elemental analysis were used to determine the chemical content and structure of the produced melanin. The melanin synthesis pathway was investigated using melanin inhibitors and by performing LC-MS analysis of the fermentation medium in which <i>S. musae</i> grew. It was found that the newly produced melanin from <i>S. musae</i> possesses antioxidant and photoprotective properties (sun protection and UV protection for <i>Staphylococcus aureus</i>). These results provide new possibilities for the biotechnological applications of this naturally producing melanin pigment.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 4","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of melanin from the fungus Scolecobasidium Musae and its antioxidant and photoprotective properties\",\"authors\":\"Fatma Meryem Bozhuyuk, Murat Ozdal\",\"doi\":\"10.1007/s00203-025-04279-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Melanin is a dark colored pigment produced by various living organisms. In this study, the melanin-producing fungus was isolated from a washing machine and identified as <i>Scolecobasidium musae</i> by 18 S rDNA analysis. Melanin production by <i>S. musae</i> was reported for the first time. Melanin was obtained from the dark-colored biomass produced by <i>S. musae</i> in submerged culture. The pigment was then purified, characterized, and identified. It was determined that purified melanin was dissolved easily in alkaline solutions but insoluble in organic solvents. Devices such as UV-Vis spectroscopy, XRD, FTIR, NMR, EPR, and elemental analysis were used to determine the chemical content and structure of the produced melanin. The melanin synthesis pathway was investigated using melanin inhibitors and by performing LC-MS analysis of the fermentation medium in which <i>S. musae</i> grew. It was found that the newly produced melanin from <i>S. musae</i> possesses antioxidant and photoprotective properties (sun protection and UV protection for <i>Staphylococcus aureus</i>). These results provide new possibilities for the biotechnological applications of this naturally producing melanin pigment.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":8279,\"journal\":{\"name\":\"Archives of Microbiology\",\"volume\":\"207 4\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-03-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00203-025-04279-2\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-025-04279-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Characterization of melanin from the fungus Scolecobasidium Musae and its antioxidant and photoprotective properties
Melanin is a dark colored pigment produced by various living organisms. In this study, the melanin-producing fungus was isolated from a washing machine and identified as Scolecobasidium musae by 18 S rDNA analysis. Melanin production by S. musae was reported for the first time. Melanin was obtained from the dark-colored biomass produced by S. musae in submerged culture. The pigment was then purified, characterized, and identified. It was determined that purified melanin was dissolved easily in alkaline solutions but insoluble in organic solvents. Devices such as UV-Vis spectroscopy, XRD, FTIR, NMR, EPR, and elemental analysis were used to determine the chemical content and structure of the produced melanin. The melanin synthesis pathway was investigated using melanin inhibitors and by performing LC-MS analysis of the fermentation medium in which S. musae grew. It was found that the newly produced melanin from S. musae possesses antioxidant and photoprotective properties (sun protection and UV protection for Staphylococcus aureus). These results provide new possibilities for the biotechnological applications of this naturally producing melanin pigment.
期刊介绍：
Research papers must make a significant and original contribution to
microbiology and be of interest to a broad readership. The results of any
experimental approach that meets these objectives are welcome, particularly
biochemical, molecular genetic, physiological, and/or physical investigations into
microbial cells and their interactions with their environments, including their eukaryotic hosts.
Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published.
Theoretical papers and those that report on the analysis or ''mining'' of data are
acceptable in principle if new information, interpretations, or hypotheses
emerge.
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