在离体培养之前,在离地圈养条件下自然感染了Inui疟原虫的长尾猕猴的血液学特征

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES
Risqa Novita, Agik Suprayogi, Andria Agusta, Arifin Budiman Nugraha, Huda Shalahudin Darusman
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引用次数: 0

摘要

以低血红蛋白为特征的贫血是疟疾的一个关键指标。非人类灵长类动物中的疟疾通常无症状,需要实验室检查和显微镜检查才能诊断。血液学资料对于了解NHP临床状况和支持体外疟原虫培养抗疟疾检测至关重要。本研究检测了自然感染猪疟原虫的猕猴的血液学变化,并为离体培养提供了血细胞比容的指导。方法对2013年确诊为犬疟原虫的10只束状支原体进行镜检和分子分析。对2只寄生率最高的猕猴(M4、M10)和1只阴性对照(M6)进行分析。测量血液学、白蛋白和葡萄糖水平。结果M4和M10红细胞压积值分别为34.5%和30.2%,血红蛋白水平分别为10.5和8.6 g/dL。M10表现为贫血和血小板减少症。结论M10表现为疟疾血液学征象,而所有猕猴均表现为小细胞性低色度贫血。体外培养推荐高红细胞压积水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hematological Profile of Naturally Infected Long-Tailed Macaques (Macaca fascicularis) With Plasmodium Inui in Ex Situ Captivity Prior to Ex Vivo Cultivation

Background

Anemia, characterized by low hemoglobin, is a key malaria indicator. Malaria in non-human primates (NHPs) is often asymptomatic, requiring laboratory tests and microscopy for diagnosis. Hematological profiles are crucial for understanding NHP clinical conditions and supporting ex vivo Plasmodium culture for antimalarial testing. This study examines hematological changes in Macaca fascicularis naturally infected with Plasmodium inui and provides hematocrit guidelines for ex vivo culture.

Methods

Ten M. fascicularis were diagnosed with Plasmodium inui in 2023 and subsequently re-screened in this study through microscopic and molecular analyses. Two monkeys (M4, M10) with the highest parasitemia and one negative control (M6) were analyzed. Hematological, albumin, and glucose levels were measured.

Results

M4 and M10 showed 34.5% and 30.2% hematocrit values, with hemoglobin levels of 10.5 and 8.6 g/dL. M10 exhibited anemia and thrombocytopenia.

Conclusions

M10 showed hematological signs of malaria, while all macaques exhibited microcytic hypochromic anemia. High hematocrit levels are recommended for ex vivo culture.

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来源期刊
CiteScore
1.40
自引率
42.90%
发文量
62
审稿时长
6 months
期刊介绍: The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.
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