YAP1通过促进牛子宫内膜上皮细胞的质膜转化和增殖来调节子宫内膜容受性

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-02-22 DOI:10.1016/j.theriogenology.2025.02.017

Han Zhou, Jinxin Zhang, Wen Feng, Nuoer Chen, Talha Umar, Xinyu Feng, Wenjing Liu, Changwei Qiu, Ganzhen Deng

{"title":"YAP1通过促进牛子宫内膜上皮细胞的质膜转化和增殖来调节子宫内膜容受性","authors":"Han Zhou, Jinxin Zhang, Wen Feng, Nuoer Chen, Talha Umar, Xinyu Feng, Wenjing Liu, Changwei Qiu, Ganzhen Deng","doi":"10.1016/j.theriogenology.2025.02.017","DOIUrl":null,"url":null,"abstract":"<div><div>Plasma membrane transformation (PMT) during early pregnancy (EP) is a widely observed phenomenon across species, but its role in cows remains understudied. Yes1-associated transcriptional regulator (YAP1) plays a vital role in human embryo implantation and uterine development. However, its relationship with PMT in bovine endometrium remains unclear. This study aims to explore the potential influence of YAP1 on PMT and the proliferation of endometrial epithelial cells. We observed PMT-related changes in the endometrium of cows during EP, characterized by reduced morphometry scores and increased fully developed pinopodes. The epithelial marker cadherin-1 (CDH1, also known as E-cadherin) expression decreased, while the mesenchymal marker vimentin (VIM) expression increased. Additionally, YAP1 expression was up-regulated and activated in the endometrial epithelium during EP. Interferon-tau (IFNT) potentially promoted PMT-related changes and activated YAP1. The knockdown of YAP1 diminished the effect of IFNT on PMT. At the same time, overexpression of YAP1 might enhance PMT-related changes and promote the proliferation, migration, and invasion ability of bovine endometrial epithelial cells (bEECs). Our findings suggest that YAP1 is activated during EP and may regulate endometrial receptivity by promoting PMT and cell proliferation.</div></div>","PeriodicalId":23131,"journal":{"name":"Theriogenology","volume":"237 ","pages":"Pages 166-177"},"PeriodicalIF":2.4000,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"YAP1 regulates endometrial receptivity by promoting the plasma membrane transformation and proliferation of bovine endometrial epithelial cells\",\"authors\":\"Han Zhou, Jinxin Zhang, Wen Feng, Nuoer Chen, Talha Umar, Xinyu Feng, Wenjing Liu, Changwei Qiu, Ganzhen Deng\",\"doi\":\"10.1016/j.theriogenology.2025.02.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Plasma membrane transformation (PMT) during early pregnancy (EP) is a widely observed phenomenon across species, but its role in cows remains understudied. Yes1-associated transcriptional regulator (YAP1) plays a vital role in human embryo implantation and uterine development. However, its relationship with PMT in bovine endometrium remains unclear. This study aims to explore the potential influence of YAP1 on PMT and the proliferation of endometrial epithelial cells. We observed PMT-related changes in the endometrium of cows during EP, characterized by reduced morphometry scores and increased fully developed pinopodes. The epithelial marker cadherin-1 (CDH1, also known as E-cadherin) expression decreased, while the mesenchymal marker vimentin (VIM) expression increased. Additionally, YAP1 expression was up-regulated and activated in the endometrial epithelium during EP. Interferon-tau (IFNT) potentially promoted PMT-related changes and activated YAP1. The knockdown of YAP1 diminished the effect of IFNT on PMT. At the same time, overexpression of YAP1 might enhance PMT-related changes and promote the proliferation, migration, and invasion ability of bovine endometrial epithelial cells (bEECs). Our findings suggest that YAP1 is activated during EP and may regulate endometrial receptivity by promoting PMT and cell proliferation.</div></div>\",\"PeriodicalId\":23131,\"journal\":{\"name\":\"Theriogenology\",\"volume\":\"237 \",\"pages\":\"Pages 166-177\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-02-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Theriogenology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0093691X25000639\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"REPRODUCTIVE BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Theriogenology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0093691X25000639","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

早孕期间的质膜转化（PMT）是一种广泛观察到的跨物种现象，但其在奶牛中的作用仍未得到充分研究。yes1相关转录调节因子（YAP1）在人类胚胎着床和子宫发育中起着至关重要的作用。然而，其与牛子宫内膜PMT的关系尚不清楚。本研究旨在探讨YAP1对PMT及子宫内膜上皮细胞增殖的潜在影响。我们观察到EP期间奶牛子宫内膜的pmt相关变化，其特征是形态测量评分降低，足部发育完全。上皮标记物cadherin-1 （CDH1，也称E-cadherin）表达降低，而间质标记物vimentin （VIM）表达升高。此外，在EP期间，子宫内膜上皮中YAP1的表达上调和激活。干扰素-tau （IFNT）可能促进pmt相关的变化并激活YAP1。YAP1的下调降低了IFNT对PMT的作用。同时，YAP1的过表达可能会增强pmt相关的变化，促进牛子宫内膜上皮细胞（bEECs）的增殖、迁移和侵袭能力。我们的研究结果表明，YAP1在EP期间被激活，并可能通过促进PMT和细胞增殖来调节子宫内膜容受性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

YAP1 regulates endometrial receptivity by promoting the plasma membrane transformation and proliferation of bovine endometrial epithelial cells

Plasma membrane transformation (PMT) during early pregnancy (EP) is a widely observed phenomenon across species, but its role in cows remains understudied. Yes1-associated transcriptional regulator (YAP1) plays a vital role in human embryo implantation and uterine development. However, its relationship with PMT in bovine endometrium remains unclear. This study aims to explore the potential influence of YAP1 on PMT and the proliferation of endometrial epithelial cells. We observed PMT-related changes in the endometrium of cows during EP, characterized by reduced morphometry scores and increased fully developed pinopodes. The epithelial marker cadherin-1 (CDH1, also known as E-cadherin) expression decreased, while the mesenchymal marker vimentin (VIM) expression increased. Additionally, YAP1 expression was up-regulated and activated in the endometrial epithelium during EP. Interferon-tau (IFNT) potentially promoted PMT-related changes and activated YAP1. The knockdown of YAP1 diminished the effect of IFNT on PMT. At the same time, overexpression of YAP1 might enhance PMT-related changes and promote the proliferation, migration, and invasion ability of bovine endometrial epithelial cells (bEECs). Our findings suggest that YAP1 is activated during EP and may regulate endometrial receptivity by promoting PMT and cell proliferation.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.