香蕉茎对病原菌的易感性及其毒力评估

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2025-02-20 DOI:10.1016/j.mex.2025.103244

T. Desmarez , P. Brat , L. Lassois , B. Barral , O. Hubert

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引用次数: 0

摘要

本议定书的目的是评估(a)真菌对香蕉茎的毒力和(b)香蕉茎切割方式/品种对病原体的敏感性。介绍了该方法的原理、使用的植物材料、持续时间和预期效果。详细介绍了材料和5个步骤：秸秆取样、接种和植物材料制备、病原体接种、培养和秸秆坏死评价。通过测定香蕉茎的坏死比例，确定接种毒力和香蕉茎对病原菌的敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Assessing banana stalk susceptibility to pathogens and their virulence

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Assessing banana stalk susceptibility to pathogens and their virulence

The purpose of this protocol is to assess (a) the virulence of fungi on banana stalks and (b) the susceptibility of a banana stalk cutting modality/cultivar to a pathogen. The principle, plant material used, duration and expected results are presented. The materials and the five procedural steps—stalk sampling, inoculum and plant material preparation, pathogen inoculation, incubation, and evaluation of stalk necrosis—are detailed. Inoculum virulence and banana stalk susceptibility to pathogenic fungi are determined by measuring the proportion of necrosis.

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