[CSF1基因cpg位点甲基化作为其表达调控因子和人类生物衰老的标志物]。

Q3 Medicine
A D Sergeeva, A D Perenkov, M V Vedunova
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引用次数: 0

摘要

年龄相关的甲基化模式转化被认为是人类生物年龄的重要预测因子。cpg二核苷酸甲基化水平的变化有助于许多基因功能的改变,包括那些与免疫系统功能相关的基因。其中一个基因就是CSF1。该基因的蛋白产物与炎症性衰老有关,使其成为年龄相关疾病的重要生物标志物。我们利用MALDI-TOF质谱法研究了CSF1基因启动子相关CpG岛的甲基化谱。在研究区域内cpg位点甲基化特征与不同年龄组人群中该基因mRNA及其蛋白产物的相对水平之间的依赖关系被寻求。对于两个CpG位点,显示出与所研究参数的高度相关性。对与基因转录相关的转录因子结合位点的着陆点的搜索表明,这些CpG二核苷酸是NFI家族转录因子和EGR1因子的基序的一部分。我们推测这些CpG位点在CSF1基因表达调控中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Methylation of Selected CpG-Sites of the Gene CSF1 as a Factor in Regulation of Its Expression and a Marker of Human Biological Aging].

Age-associated transformation of methylation patterns is considered to be an important predictor of human biological age. Changes in the level of CpG-dinucleotide methylation contribute to a shift in the function of a number of genes, including those associated with the functioning of the immune system. One such gene is CSF1. The protein product of this gene is associated with inflammatory aging, making it an important biomarker of age-related diseases. We studied the methylation profile of the promoter-associated CpG island of the CSF1 gene by MALDI-TOF mass spectrometry. Dependences between the character of CpG-site methylation within the investigated regions and the relative level of the gene mRNA and its protein product in people of different age groups were sought. For two CpG sites, a high level of correlation with the studied parameters is shown. A search for the landing sites of transcription factor binding sites associated with gene transcription showed that these CpG dinucleotides are part of motifs for the NFI family transcription factors and the EGR1 factor. We hypothesize that these CpG sites play an important role in the regulation of CSF1 gene expression.

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来源期刊
Molekulyarnaya Biologiya
Molekulyarnaya Biologiya Medicine-Medicine (all)
CiteScore
0.70
自引率
0.00%
发文量
131
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