Garry J. Rucklidge , Gwen I. Riddoch , Lynda M. Williams , Simon P. Robins
{"title":"大鼠肾清除循环I型胶原碎片的放射自显影研究","authors":"Garry J. Rucklidge , Gwen I. Riddoch , Lynda M. Williams , Simon P. Robins","doi":"10.1016/S0174-173X(88)80005-0","DOIUrl":null,"url":null,"abstract":"<div><p>Cyanogen bromide (CNBr) peptides of <sup>3</sup>H-labelled type I collagen isolated fromyoung rat skin were injected into adult rats via the tail vein. After 1, 5 and 10 minutes, the fate of the circulating radioactive peptides was investigated by measurement of the tissue distribution of radioactivity. Rapid removal of peptides from the circulation and their concomitant accumulation in the kidney proximal tubule epithelia was confirmed by autoradiography of kidney sections at the light microscope level. Immunopositive material was also identified and localized in proximal tubules using antibodies directed against denatured type I collagen chains and their CNBr degradation products. Ultrastructural autoradiography indicated that rapid removal of collagen peptides from the glomerular filtrate occurs via the brush border, apical vacuoles, and lysosomes.</p></div>","PeriodicalId":77694,"journal":{"name":"Collagen and related research","volume":"8 4","pages":"Pages 339-348"},"PeriodicalIF":0.0000,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-173X(88)80005-0","citationCount":"15","resultStr":"{\"title\":\"Autoradiographic Studies of the Renal Clearance of Circulating Type I Collagen Fragments in the Rat\",\"authors\":\"Garry J. Rucklidge , Gwen I. Riddoch , Lynda M. Williams , Simon P. Robins\",\"doi\":\"10.1016/S0174-173X(88)80005-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Cyanogen bromide (CNBr) peptides of <sup>3</sup>H-labelled type I collagen isolated fromyoung rat skin were injected into adult rats via the tail vein. After 1, 5 and 10 minutes, the fate of the circulating radioactive peptides was investigated by measurement of the tissue distribution of radioactivity. Rapid removal of peptides from the circulation and their concomitant accumulation in the kidney proximal tubule epithelia was confirmed by autoradiography of kidney sections at the light microscope level. Immunopositive material was also identified and localized in proximal tubules using antibodies directed against denatured type I collagen chains and their CNBr degradation products. Ultrastructural autoradiography indicated that rapid removal of collagen peptides from the glomerular filtrate occurs via the brush border, apical vacuoles, and lysosomes.</p></div>\",\"PeriodicalId\":77694,\"journal\":{\"name\":\"Collagen and related research\",\"volume\":\"8 4\",\"pages\":\"Pages 339-348\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0174-173X(88)80005-0\",\"citationCount\":\"15\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Collagen and related research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0174173X88800050\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Collagen and related research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0174173X88800050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Autoradiographic Studies of the Renal Clearance of Circulating Type I Collagen Fragments in the Rat
Cyanogen bromide (CNBr) peptides of 3H-labelled type I collagen isolated fromyoung rat skin were injected into adult rats via the tail vein. After 1, 5 and 10 minutes, the fate of the circulating radioactive peptides was investigated by measurement of the tissue distribution of radioactivity. Rapid removal of peptides from the circulation and their concomitant accumulation in the kidney proximal tubule epithelia was confirmed by autoradiography of kidney sections at the light microscope level. Immunopositive material was also identified and localized in proximal tubules using antibodies directed against denatured type I collagen chains and their CNBr degradation products. Ultrastructural autoradiography indicated that rapid removal of collagen peptides from the glomerular filtrate occurs via the brush border, apical vacuoles, and lysosomes.
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