马来西亚植物病原菌Pantoea ananatis新型噬菌体PA-1的全基因组序列分析

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-02-17 DOI:10.1007/s00705-025-06239-z

Pritpal Singh Sarmak Singh, Wai Keat Toh, Boon Hoe Lim, Hann Ling Wong, Pek Chin Loh

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引用次数: 0

摘要

一种新的噬菌体PA-1，是用Pantoea ananatis作为宿主分离出来的，Pantoea ananatis是一种世界范围内导致水稻叶枯病的细菌。PA-1具有46,332 bp的线性基因组，GC含量为50.55%。包含83个蛋白编码基因，未检测到trna编码基因。基因组组织密集，平均CDS大小为516 bp， 92.38%的基因组由蛋白质编码区组成。全基因组测序、系统发育和形态分析表明，PA-1是一种新的噬菌体，在系统发育上是独特的，表明它可能代表了尾柱菌纲的一个新属。

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Complete genome sequence analysis of the novel bacteriophage PA-1 infecting phytopathogenic Pantoea ananatis in Malaysia

A novel bacteriophage, PA-1, was isolated using Pantoea ananatis, a bacterium that is responsible for rice leaf blight worldwide, as the host. PA-1 has a linear genome of 46,332 bp with a GC content of 50.55%. It contains 83 protein-encoding genes, and no tRNA-encoding genes were detected. The genome is densely organized, with an average CDS size of 516 bp, and 92.38% of the genome consists of protein-coding regions. Whole-genome sequencing and phylogenetic and morphological analysis showed that PA-1 is a novel phage that is phylogenetically distinct, suggesting that it may represent a new genus within the class Caudoviricetes.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.