IF 4.5 2区 医学 Q2 IMMUNOLOGY
Yu-Lin Lee , Chun-Eng Liu , Wei-Yao Wang , Mei-Chen Tan , Pei-Jing Chen , Yih-Ru Shiau , Hui-Ying Wang , Jui-Fen Lai , I-Wen Huang , Ya-Sung Yang , Shu-Chen Kuo
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引用次数: 0

摘要

背景:研究台湾2020-2022年国家监测项目收集的亚胺培南非敏感大肠埃希菌(ens - ec)和肺炎克雷伯菌(ens - kp)分离株对新型抗生素的敏感性,并与2012-2018年的结果进行比较。方法:采用微量肉汤稀释法测定最低抑菌浓度。通过多重PCR检测碳青霉烯酶编码基因,包括blaKPC、金属β-内酰胺酶(MBL)基因和blaOXA-48。我们从2012-2018年的研究中检索数据进行比较。结果:2020-2022年收集的3260株大肠杆菌和1457株肺炎克雷伯菌中,分别有0.9%和9.5%为INS-EC和INS-KP。头孢吡肟-齐德巴坦、头孢他啶-阿维巴坦、亚胺培南-勒布巴坦和美罗培尼-瓦波巴坦对29株INS-EC的活性分别为100%、75.9%、65.5%和79.3%;138株INS- KP分别为100%、90.6%、64.5%和67.4%。敏感性取决于碳青霉烯酶类型。2012 - 2018年至2020-2022年,头孢吡肟-齐德巴坦和头孢他啶-阿维巴坦的易感率保持不变,但亚胺培南-勒巴坦和美罗培宁-瓦波巴坦的易感率明显下降,这可能与blaOXA-48的患病率上升有关。18株mbl基因阳性菌株和2株blakpc阳性菌株对头孢他啶-阿维巴坦耐药,而对头孢吡肟-齐德巴坦均敏感。替加环素对肺炎克雷伯菌的敏感性高于依拉瓦环素和奥马达环素。拉库沙星和德拉沙星对不到10%的INS分离株有效。从2012 - 2018年到2020-2022年,对新型四环素类药物和氟喹诺酮类药物的敏感性保持相似。结论:本研究突出了台湾地区INS-EC和INS-KP分离株的显著耐药模式。遗传耐药决定因素的易感性下降和流行率上升突出了持续监测和抗微生物药物管理的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial resistance among imipenem-non-susceptible Escherichia coli and Klebsiella pneumoniae isolates, with an emphasis on novel β-lactam/β-lactamase inhibitor combinations and tetracycline derivatives: The Taiwan surveillance of antimicrobial resistance program, 2020–2022

Background

To determine susceptibility of imipenem-non-susceptible Escherichia coli (INS-EC) and Klebsiella pneumoniae (INS-KP) isolates collected during 2020–2022 through a national surveillance program in Taiwan to novel antibiotics, and to compare the results with those obtained during 2012–2018.

Methods

Minimum inhibitory concentrations were determined by broth microdilution methods. Genes encoding carbapenemases including blaKPC, metallo-β-lactamase (MBL) genes, and blaOXA-48 were detected via multiplex PCR. Data retrieved from our 2012–2018 study were used for comparison.

Results

Of 3260 E. coli and 1457 K. pneumoniae isolates collected during 2020–2022, 0.9 % and 9.5 %, were INS-EC and INS-KP, respectively. Cefepime-zidebactam, ceftazidime-avibactam, imipenem-relebactam, and meropenem-vaborbactam were active against 100 %, 75.9 %, 65.5 %, and 79.3 % of 29 INS-EC isolates respectively; and against 100 %, 90.6 %, 64.5 %, and 67.4 % of 138 INS- KP isolates, respectively. Susceptibility was contingent upon carbapenemase types. Susceptibility rates of cefepime-zidebactam and ceftazidime-avibactam remained constant from 2012 to 2018 through 2020–2022 but those of imipenem-relebactam and meropenem-vaborbactam decreased significantly, which may be partially attributable to the increasing prevalence of blaOXA-48. Eighteen MBL-gene-positive isolates and two blaKPC-positive isolates were resistant to ceftazidime-avibactam, whereas all were susceptible to cefepime-zidebactam. Tigecycline had a higher susceptibility rate than eravacycline and omadacycline for K. pneumoniae isolates. Lascufloxacin and delafloxacin were effective against fewer than 10 % of INS isolates. Susceptibilities to novel tetracyclines and fluoroquinolones remained similar from 2012 to 2018 through 2020–2022.

Conclusions

This study highlights significant resistance patterns of INS-EC and INS-KP isolates in Taiwan. The declining susceptibility rates and the rising prevalence of genetic resistance determinants highlight the importance of ongoing surveillance and antimicrobial stewardship.
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来源期刊
Journal of Microbiology Immunology and Infection
Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES
CiteScore
15.90
自引率
5.40%
发文量
159
审稿时长
67 days
期刊介绍: Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.
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