在Dunkin-Hartley骨关节炎模型中,短期手工针刺可降低全身炎症标志物和改变关节软骨转录物。

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
American journal of veterinary research Pub Date : 2025-02-07 Print Date: 2025-04-01 DOI:10.2460/ajvr.24.11.0341
Alexa P Spittler, Katherine E Bukovec, Maryam F Afzali, Sarah E Leavell, Sydney B Bork, Cassie A Seebart, Kelly S Santangelo, Melinda R Story
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引用次数: 0

摘要

目的:探讨针刺治疗Dunkin-Hartley豚鼠(Cavia porcellus)的活动能力、炎症和软骨重塑指标与2个不同比较针刺组的差异。方法:选取12月龄雄性哈特利犬,随机分为3组,分别采用手针刺、针鞘轻击相应穴位和离穴针刺。异氟醚处理每周一次,连续3周,并以相同频率进行野外封闭监测。最终治疗结束后,对所有动物实施安乐死,采集血液进行炎症标志物分析,收集组织进行组织学、免疫组织化学和转录物表达分析。结果:共18只动物,每实验组6只。针刺组血清补体成分3、前列腺素E2浓度显著降低(P < 0.05)。与对照组相比,手针刺组胃-36穴位肌肉有6个基因转录本表达改变。从软骨/半月板,手工针刺导致13个基因转录本下调。胫骨内侧平台关节软骨3层神经生长因子(NGF)免疫染色结果显示,针刺组明显高于对照组。围封监测参数和组织学分级无差异。结论:未见明显的自主活动能力、行为或血清生化参数或窒息组织结构的变化。分析了血清炎症蛋白、软骨重塑转录物基因表达和软骨中NGF蛋白浓度的差异。临床意义:短时间的手针刺显示了有益的再生和重塑过程的启动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Short-term manual acupuncture decreased markers of systemic inflammation and altered articular cartilage transcripts in the Dunkin-Hartley model of osteoarthritis.

Objective: To investigate indicators of mobility, inflammation, and cartilage remodeling in Dunkin-Hartley guinea pigs (Cavia porcellus) treated with manual acupuncture compared to 2 different comparator acupuncture groups.

Methods: 12-month-old male Hartleys were randomly assigned to 1 of 3 in vivo experimental groups that received manual acupuncture, needle sheath taps on corresponding acupoints, or off-point acupuncture. Treatments were performed under isoflurane once weekly for 3 weeks, and open-field enclosure monitoring was performed at the same frequency. After final treatments, all animals were euthanized, blood was collected for inflammatory marker analysis, and tissues were collected for histology, immunohistochemistry, and transcript expression analysis.

Results: 18 animals were involved: 6 per experimental group. Serum concentrations of complement component 3 and prostaglandin E2 were significantly decreased in the acupuncture group (P < .05). Muscle from acupoint stomach-36 had 6 gene transcripts with altered expressions in the manual acupuncture group compared to comparators. From cartilage/menisci, manual acupuncture resulted in the downregulation of 13 gene transcripts. Nerve growth factor (NGF) immunostaining in all 3 layers of articular cartilage of the medial tibial plateau was greater in the manual acupuncture group relative to the comparator groups. There were no differences in enclosure monitoring parameters or histologic grading.

Conclusions: Appreciable changes in voluntary mobility, behavioral or serum biochemical parameters, or stifle histological structure were not seen. Differences in serum inflammatory proteins, the gene expression of cartilage-remodeling transcripts, and NGF protein concentrations in cartilage were elucidated.

Clinical relevance: The short duration of manual acupuncture showed the initiation of beneficial regenerative and remodeling processes.

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来源期刊
CiteScore
1.70
自引率
10.00%
发文量
186
审稿时长
3 months
期刊介绍: The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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