{"title":"一种基于转录因子的生物传感器，用于鉴定聚对苯二甲酸乙二醇酯降解酶","authors":"Shengwei Sun","doi":"10.1016/j.inv.2025.100038","DOIUrl":null,"url":null,"abstract":"<div><div>Screening and mining efficient polyethylene terephthalate (PET)-degrading enzyme is a promising strategy for plastic waste treatment and recycling. This invention provides a novel transcription factor-based biosensor method for identifying potential PET-degrading enzymes from the microorganisms. When PET is degraded by a specific enzyme to produce terephthalic acid (TPA), the transcription factor TphR recognizes the TPA substrate and then activates the transcription of downstream green fluorescent protein (GFP) reporter, generating measurable fluorescence signals in bacterial cells.</div></div>","PeriodicalId":100728,"journal":{"name":"Invention Disclosure","volume":"5 ","pages":"Article 100038"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A transcription factor-based biosensor for identification of the polyethylene terephthalate-degrading enzyme\",\"authors\":\"Shengwei Sun\",\"doi\":\"10.1016/j.inv.2025.100038\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Screening and mining efficient polyethylene terephthalate (PET)-degrading enzyme is a promising strategy for plastic waste treatment and recycling. This invention provides a novel transcription factor-based biosensor method for identifying potential PET-degrading enzymes from the microorganisms. When PET is degraded by a specific enzyme to produce terephthalic acid (TPA), the transcription factor TphR recognizes the TPA substrate and then activates the transcription of downstream green fluorescent protein (GFP) reporter, generating measurable fluorescence signals in bacterial cells.</div></div>\",\"PeriodicalId\":100728,\"journal\":{\"name\":\"Invention Disclosure\",\"volume\":\"5 \",\"pages\":\"Article 100038\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Invention Disclosure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772444125000023\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Invention Disclosure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772444125000023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A transcription factor-based biosensor for identification of the polyethylene terephthalate-degrading enzyme
Screening and mining efficient polyethylene terephthalate (PET)-degrading enzyme is a promising strategy for plastic waste treatment and recycling. This invention provides a novel transcription factor-based biosensor method for identifying potential PET-degrading enzymes from the microorganisms. When PET is degraded by a specific enzyme to produce terephthalic acid (TPA), the transcription factor TphR recognizes the TPA substrate and then activates the transcription of downstream green fluorescent protein (GFP) reporter, generating measurable fluorescence signals in bacterial cells.
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