雌激素对雌雄大鼠心肌缺血再灌注损伤的影响及机制。

IF 2.1 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Steroids Pub Date : 2025-02-01 DOI:10.1016/j.steroids.2025.109561

Sichong Chen , Lijuan Yang , Jiayao Xue , Xinmiao Tian, Huiyuan Hu, Qinghua Gao, Rui Feng, Liying Hao

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引用次数: 0

摘要

由于不同发情周期雌激素水平的差异，在研究雌激素及其类似物的心血管作用时，有必要排除内源性雌激素的影响。本研究对雌性大鼠在不同发情周期的离体心脏缺血再灌注（I/R）损伤进行研究，并与雄性大鼠进行比较。结果表明，大鼠在初潮和发情（MD）期间血液中雌激素含量低于前情和发情（PE）期间。10-8 M的17β-雌二醇（E2）对PE过程中雄性大鼠和雌性大鼠I/R损伤无显著影响。E2对心肌梗死期间雌性大鼠的心率（HR）、乳酸脱氢酶（LDH）和肌酸激酶（CK）释放均有明显的保护作用，E2通过降低心肌梗死面积和p-CaMKII表达水平减轻心肌梗死期间雌性大鼠的I/R损伤。提示发情周期可能通过E2对CaMKII表达水平的调节而影响心脏I/R损伤程度，提示在雌激素和环境激素研究中，应考虑发情周期对动物模型制备和毒性研究的影响。

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Effect of estrogen on myocardial ischemia–reperfusion injury in male and female rats and related mechanism

Due to the difference of estrogen levels in different phases of estrous cycle, it is necessary to exclude the influence of endogenous estrogen when studying the cardiovascular effects of estrogen and its analogues. In this study, the ischemia/reperfusion (I/R) injury of isolated heart were investigated in female rats during different phases of estrous cycle with male rats as comparison. The results indicated that the estrogen content in blood of rats during metestrus and diestrus (MD) was lower than those during proestrus and estrous (PE). 17β-Estradiol (E2) at 10⁻⁸ M did not show significant effects on I/R injury in male rats and female rats during PE. However, E2 exerted an obviously protective effects against I/R injury on heart rate (HR), lactate dehydrogenase (LDH) and creatine kinase (CK) release in female rats during MD. Furthermore, E2 relieved I/R injury in female rats during MD by decreasing the infarct size and the expression level of p-CaMKII. The results suggested estrous cycles may influence on the extent of cardiac I/R injury due to the regulation of E2 on CaMKII expression level, providing an idea that the estrus cycle should be considered for animal model preparation and toxicity studies in estrogen and environmental hormone research.

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来源期刊

Steroids 医学-内分泌学与代谢

CiteScore

5.10

自引率

3.70%

发文量

120

审稿时长

73 days

期刊介绍： STEROIDS is an international research journal devoted to studies on all chemical and biological aspects of steroidal moieties. The journal focuses on both experimental and theoretical studies on the biology, chemistry, biosynthesis, metabolism, molecular biology, physiology and pharmacology of steroids and other molecules that target or regulate steroid receptors. Manuscripts presenting clinical research related to steroids, steroid drug development, comparative endocrinology of steroid hormones, investigations on the mechanism of steroid action and steroid chemistry are all appropriate for submission for peer review. STEROIDS publishes both original research and timely reviews. For details concerning the preparation of manuscripts see Instructions to Authors, which is published in each issue of the journal.