Jing Guo, Xiang-Ying Ouyang, Jian-Ru Liu, Wen-Yi Liu, Yuan-Bo Wang
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引用次数: 0
摘要
背景/目的：牙周炎造成的局部炎症微环境对牙周组织的再生产生了负面影响，因此有必要开发增强干细胞再生能力的方法。本研究探讨了 miR-508-5p 在人牙周韧带干细胞（hPDLSCs）成骨分化过程中的调控作用及其内在机制：通过抑制或过表达miR-508-5p，研究miR-508-5p在hPDLSCs成骨分化中的调控作用。采用 Western 印迹和实时 PCR 分析了性别决定区 Y 相关 HMG-box 11（SOX11）和成骨标记物的表达。使用茜素红 S（ARS）染色法和碱性磷酸酶（ALP）染色法测量骨生成。为了证实SOX11是miR-508-5p的靶标，进行了双荧光素酶报告实验：结果：在hPDLSCs的成骨分化过程中，miR-508-5p的表达水平逐渐降低，而SOX11的表达水平逐渐升高。SOX11的过表达逆转了miR-508-5p对hPDLSCs成骨分化的抑制作用。miR-508-5p的下调明显增加了SOX11的表达量；双荧光素酶报告实验为它们的直接靶向提供了证据。
miR-508-5p suppresses osteogenic differentiation of human periodontal ligament stem cells via targeting sex-determining region Y-related HMG-box 11.
Background/purpose: The local inflammatory microenvironment created by periodontitis negatively impacts periodontal tissue regeneration, necessitating the development of methods to enhance the regenerative capacity of stem cells. This study explored the regulatory role and underlying mechanism of miR-508-5p in the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs).
Materials and methods: The regulatory roles of miR-508-5p in osteogenic differentiation of hPDLSCs were investigated through its inhibition or overexpression. Expression of the sex-determining region Y-related HMG-box 11 (SOX11) and osteogenic markers was analyzed using Western blot and real-time PCR. Osteogenesis was measured using alizarin red S (ARS) staining and alkaline phosphatase (ALP) staining. A dual luciferase reporter assay was performed to confirm SOX11 as a target of miR-508-5p.
Results: During the osteogenic differentiation of hPDLSCs, miR-508-5p expression level gradually decreased, while that of SOX11 increased. miR-508-5p inhibition significantly promoted osteogenesis in hPDLSCs, while overexpression inhibited the process. SOX11 overexpression reversed the suppressive effects of miR-508-5p on the osteogenic differentiation of hPDLSCs. miR-508-5p downregulation significantly increased SOX11; a dual luciferase reporter assay provided evidence for their direct targeting.
Conclusion: miR-508-5p downregulation promotes the osteogenic differentiation of hPDLSCs by targeting SOX11.
期刊介绍：
he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases.
The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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