新型小麦赤霉病菌3ANX化学型变异qPCR检测和定量方法的建立

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-02-01 DOI:10.1016/j.mimet.2025.107091

Abbey Saunders, Emily Johnstone, Adam Foster

引用次数: 0

摘要

毁灭性的植物病原体镰刀菌产生真菌毒素，包括新的3ANX毒素。为了检测产生3ANX的分离株，开发了SYBR Green和锁定核酸探针检测方法，针对3ANX Tri1多态性。检测在R2 > 0.99下有效，对3ANX具有特异性，检测到的拷贝数低至2个/μL。

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Development of qPCR methods to detect and quantify the novel Fusarium graminearum 3ANX chemotype variant

The devastating plant pathogen Fusarium graminearum produces mycotoxins including the novel 3ANX toxin. To detect 3ANX-producing isolates, SYBR Green and locked nucleic acid probe assays were developed, targeting 3ANX Tri1 polymorphisms. Assays were efficient with R² > 0.99 and specific to 3ANX, detecting as few as 2 copies/μL.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.