使用化学发生方法研究老年小鼠下丘脑和白色脂肪组织之间的组织间通讯和寿命的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-01-10 DOI:10.1016/j.xpro.2024.103551

Kyohei Tokizane, Shin-Ichiro Imai

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引用次数: 0

摘要

在这里，我们提出了一种方案，用于评估化学发生操作对下丘脑神经元亚群对衰老和寿命控制的影响，使用专门为此目的开发的小鼠模型。我们描述了立体定向病毒注射的步骤，并评估了下丘脑背内侧表达蛋白磷酸酶1调节亚基17 （Ppp1r17）的神经元和白色脂肪组织之间的组织间通讯。然后，我们详细介绍了在老年小鼠中进行化学发生操作后的寿命测量程序。关于本协议使用和执行的完整细节，请参考Tokizane et al.1。

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Protocol to study inter-tissue communication between the hypothalamus and white adipose tissue and lifespan using a chemogenetic approach in aged mice.

Here, we present a protocol for assessing the impact of a chemogenetic manipulation in a subpopulation of the hypothalamic neurons on aging and lifespan control using a mouse model developed specifically for this purpose. We describe steps for stereotaxic viral injection and assess inter-tissue communication between protein phosphatase 1 regulatory subunit 17 (Ppp1r17)-expressing neurons in the dorsomedial hypothalamus and white adipose tissue. We then detail procedures for lifespan measurements following chemogenetic manipulation in aged mice. For complete details on the use and execution of this protocol, please refer to Tokizane et al.¹.

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