基于aiegen的高选择性“开启”荧光成像用于炎症检测。

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2025-01-01 DOI:10.1002/bio.70075

Cong Shao, Xiaoxuan Wang, Jingying Dai, Honglian Dai

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引用次数: 0

摘要

次氯酸（HClO）是由免疫系统中的免疫细胞释放的，它通过杀死细菌、病毒和其他病原体来帮助身体抵抗感染和炎症。然而，过量的HClO也可能引起组织损伤或细胞凋亡。在此基础上，我们选择四苯基乙烯（TPE）作为发光单元，二甲基硫代氨基氯作为识别位点，设计了探针TPE- ns。通过高斯跃迁态计算，HClO会通过水解反应切断TPE-NS的光致电子转移（PET）效应，从而发出强烈的荧光。TPE-NS对HClO的识别速度快，特异性好，检出限为7.27 μM。最后，TPE-NS成功用于细胞实验中内源性和外源性HClO的可视化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Highly Selective AIEgen-Based "Turn On" Fluorescent Imaging for Inflammation Detection.

Hypochlorous acid (HClO) is released by immune cells in the immune system, and it helps the body fight off infections and inflammation by killing bacteria, viruses, and other pathogens. However, tissue damage or apoptosis may also be induced by excess HClO. On this basis, we designed the probe TPE-NS by choosing tetraphenylethylene (TPE) as the luminescent unit and dimethylthiocarbamoyl chloride as the recognition site. By Gaussian's transition state calculations, HClO will cut off the photoinduced electron transfer (PET) effect of TPE-NS by hydrolysis reaction, thus emitting strong fluorescence. TPE-NS has rapid recognition and excellent specificity for HClO, and the limit of detection is 7.27 μM. Finally, TPE-NS was successfully used for the visualization of endogenous and exogenous HClO in cell experiments.

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.