一种用催化比色反应检测食品中重金属污染的快速、多路和肉眼可读纸试验方法。

IF 3.7 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Dairy Science Pub Date : 2025-04-01 DOI:10.3168/jds.2024-25838

Ying Huang , Jianwei Wu , Wenping Yang , Qilin Qiu , Qin Liu , Jie Li , Jiangwei Wen , Wenbo Cheng , Xuhan Xia

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引用次数: 0

摘要

重金属污染是严重的食品安全问题。在此，我们报告了一种快速，多路和肉眼可读的方法，用于检测食品样品中的重金属污染，使用廉价的比色纸，包括牛奶。我们利用重金属离子Hg2+和Pb2+对脲酶的强烈抑制作用，利用脲酶催化反应来放大重金属离子Hg2+和Pb2+的存在。结合折纸策略，可在5分钟内快速检测10个以上样品，Hg2+低至0.1 nM， Pb2+低至2 μM，可通过肉眼或智能手机读取结果。结果可以用肉眼或智能手机直观地解读，使其适用于现场测试，并确保乳制品免受金属污染的安全。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A rapid, multiplexed, and naked-eye-readable paper assay for detecting heavy metal pollution in food using a catalytic colorimetric reaction

Heavy metal contamination is a serious food safety issue. Herein, we report a rapid, multiplexed, and naked-eye-readable method for detecting heavy metal pollution in food samples, including milk, using an inexpensive colorimetric paper. We leverage the urease catalysis reaction to amplify the presence of the heavy metal ions Hg²⁺ and Pb²⁺ by exploiting their strong inhibitory effect on urease. Integrating with a paper-folding strategy, the visual assay realizes rapid detection of over 10 samples within 5 min. It allowed detection of Hg²⁺ as low as 0.1 nM and Pb²⁺ as low as 2 μM. Results can be visually interpreted with the naked eye or a smartphone, making it practical for on-site testing and ensuring the safety of dairy products against metal contamination.

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来源期刊

Journal of Dairy Science 农林科学-奶制品与动物科学

CiteScore

7.90

自引率

17.10%

发文量

784

审稿时长

4.2 months

期刊介绍： The official journal of the American Dairy Science Association®, Journal of Dairy Science® (JDS) is the leading peer-reviewed general dairy research journal in the world. JDS readers represent education, industry, and government agencies in more than 70 countries with interests in biochemistry, breeding, economics, engineering, environment, food science, genetics, microbiology, nutrition, pathology, physiology, processing, public health, quality assurance, and sanitation.