一种用于性传播疾病快速检测的trago介导核酸检测系统及便携式装置。

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2024-12-20 DOI:10.1016/j.bios.2024.117029

Jiexiu Zhang , Zhongjing Su , Qiulan Luo , Huagui wei , Jiayu Liao , Weizhong chen , Jie Lin , Juntian Zhang , Shuheng Cai , Xiaozhong Wang , Min Lin

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引用次数: 0

摘要

开发快速和多路护理点诊断工具对于预防和控制性传播疾病至关重要。本研究开发了一种POC-comprehensive Thermococcus thioreducensArgonaute （trago）介导的核酸检测系统（POC-CANDY）和手掌大小的便携式检测装置“Owl-1”，用于同时检测解脲支原体、沙眼衣原体、淋病奈瑟菌、人乳头瘤病毒16/18型和抗生素耐药分子标记物[tetM， gyrA突变（S91F）]。利用重组酶聚合酶扩增（RPA）技术，优化后的POC-CANDY可在55 min内完成整个检测过程，检测限为10拷贝/μL。经临床STI样本验证，POC-CANDY与定量PCR的一致性为100%。此外，与基于pago的系统相比，POC-CANDY显著提高了区分单核苷酸变异的敏感性。结果表明，pocc - candy可以方便地在局部或现场应用。本研究也促进了ttrago介导技术在临床诊断中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

TtrAgo-mediated nucleic acid detection system and portable device for rapid detection of sexually transmitted diseases

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TtrAgo-mediated nucleic acid detection system and portable device for rapid detection of sexually transmitted diseases

The development of rapid and multiplexed point-of-care (POC) diagnostic tools is vital for the prevention and control of sexually transmitted diseases (STIs). Here, we developed a POC-comprehensive Thermococcus thioreducens Argonaute (TtrAgo)-mediated nucleic acid detection system (POC-CANDY) and palm-sized portable detection device “Owl-1” for the simultaneous detection of Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae, human papillomavirus types 16/18 and antibiotic resistance molecular markers [tetM, and gyrA mutation (S91F)]. Using recombinase polymerase amplification (RPA), the optimized POC-CANDY could finish the whole detection procedure within 55 min and achieve a limit of detection of 10 copies/μL. When validated by clinical STI samples, POC-CANDY showed 100% consistency with quantitative PCR. Additionally, compared with the PfAgo-based system, POC-CANDY significantly improved the sensitivity of distinguishing single nucleotide variations. The results demonstrated that POC-CANDY can be easily applied locally or on site. This study also promotes the utility of the TtrAgo-mediated technique in clinical diagnosis.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.