{"title":"油污染土壤中分离的一种新型柴油降解细菌——油酸Limnobacter olei sp. nov.。","authors":"Yongchuang Liu, Siqiong Xu, Jialiang Li, Yujie Ouyang, Shuai Gao, Pan Yang, Cuiwei Chu, Jian He, Tongwen Yang, Keshi Ma, Lili Li, Chao Wang","doi":"10.1007/s00284-024-04053-5","DOIUrl":null,"url":null,"abstract":"<p><p>A bacterial strain P1<sup>T</sup>, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4-45 °C (optimum at 28 °C), at pH 4.0-12.0 (optimum at pH 10.0) and with 0-15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1<sup>T</sup> was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612<sup> T</sup> (99.8%), followed by Limnobacter alexandrii LZ-4<sup> T</sup> (99.4%), Limnobacter parvus YS8-69<sup> T</sup> (98.8%), Limnobacter litoralis KP1-19<sup> T</sup> (97.6%), and Limnobacter humi UCM-39<sup> T</sup> (97.5%). The draft genome sequence of strain P1<sup>T</sup> was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1<sup>T</sup> and the closely related type strains were in the range of 71.8-85.1% and 18.1-28.7%, respectively. The predominant cellular fatty acids of strain P1<sup>T</sup> included C<sub>16: 0</sub>, summed feature 3 (C<sub>16: 1</sub> ɷ7c and/or C<sub>16: 1</sub> ɷ6c), summed feature 8 (C<sub>18: 1</sub> ɷ7c and/or C<sub>18: 1</sub> ɷ6c), and summed feature 7 (C<sub>19: 1</sub> ɷ6c and/or C<sub>19: 1</sub> ɷ7c and/or C<sub>19: 1</sub> cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1<sup>T</sup> (= KCTC 72814<sup> T</sup> = CCTCC AB 2019403<sup> T</sup>) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"82 2","pages":"69"},"PeriodicalIF":2.3000,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Limnobacter olei sp. nov., a Novel Diesel-Degrading Bacterium Isolated from Oil-Contaminated Soil.\",\"authors\":\"Yongchuang Liu, Siqiong Xu, Jialiang Li, Yujie Ouyang, Shuai Gao, Pan Yang, Cuiwei Chu, Jian He, Tongwen Yang, Keshi Ma, Lili Li, Chao Wang\",\"doi\":\"10.1007/s00284-024-04053-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A bacterial strain P1<sup>T</sup>, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4-45 °C (optimum at 28 °C), at pH 4.0-12.0 (optimum at pH 10.0) and with 0-15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1<sup>T</sup> was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612<sup> T</sup> (99.8%), followed by Limnobacter alexandrii LZ-4<sup> T</sup> (99.4%), Limnobacter parvus YS8-69<sup> T</sup> (98.8%), Limnobacter litoralis KP1-19<sup> T</sup> (97.6%), and Limnobacter humi UCM-39<sup> T</sup> (97.5%). The draft genome sequence of strain P1<sup>T</sup> was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1<sup>T</sup> and the closely related type strains were in the range of 71.8-85.1% and 18.1-28.7%, respectively. The predominant cellular fatty acids of strain P1<sup>T</sup> included C<sub>16: 0</sub>, summed feature 3 (C<sub>16: 1</sub> ɷ7c and/or C<sub>16: 1</sub> ɷ6c), summed feature 8 (C<sub>18: 1</sub> ɷ7c and/or C<sub>18: 1</sub> ɷ6c), and summed feature 7 (C<sub>19: 1</sub> ɷ6c and/or C<sub>19: 1</sub> ɷ7c and/or C<sub>19: 1</sub> cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1<sup>T</sup> (= KCTC 72814<sup> T</sup> = CCTCC AB 2019403<sup> T</sup>) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.</p>\",\"PeriodicalId\":11360,\"journal\":{\"name\":\"Current Microbiology\",\"volume\":\"82 2\",\"pages\":\"69\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-01-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00284-024-04053-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-04053-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Limnobacter olei sp. nov., a Novel Diesel-Degrading Bacterium Isolated from Oil-Contaminated Soil.
A bacterial strain P1T, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4-45 °C (optimum at 28 °C), at pH 4.0-12.0 (optimum at pH 10.0) and with 0-15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1T was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612 T (99.8%), followed by Limnobacter alexandrii LZ-4 T (99.4%), Limnobacter parvus YS8-69 T (98.8%), Limnobacter litoralis KP1-19 T (97.6%), and Limnobacter humi UCM-39 T (97.5%). The draft genome sequence of strain P1T was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1T and the closely related type strains were in the range of 71.8-85.1% and 18.1-28.7%, respectively. The predominant cellular fatty acids of strain P1T included C16: 0, summed feature 3 (C16: 1 ɷ7c and/or C16: 1 ɷ6c), summed feature 8 (C18: 1 ɷ7c and/or C18: 1 ɷ6c), and summed feature 7 (C19: 1 ɷ6c and/or C19: 1 ɷ7c and/or C19: 1 cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1T (= KCTC 72814 T = CCTCC AB 2019403 T) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.
期刊介绍：
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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