{"title":"住院鲍曼不动杆菌分离株blaOXA-51-23-58、blaVIM、blaNDM和blaIMP碳青霉烯酶基因的鉴定","authors":"Kosar Salmani Nasrabadi, Yasaman Ahmadi, Seyed Mahdi Ghasemi, Dariush Shokri","doi":"10.1186/s13104-024-07047-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.</p><p><strong>Results: </strong>In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla<sub>OXA-51</sub>, bla<sub>OXA-23</sub>, bla<sub>OXA-58</sub>, bla<sub>NDM</sub>, bla<sub>IMP</sub>, and bla<sub>VIM</sub> genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla<sub>OXA-23</sub> gene, 23 (92.0%) contained the bla<sub>OXA-51</sub> gene, and 1 (4.0%) isolate carried the bla<sub>NDM</sub> gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla<sub>OXA-51</sub> and bla<sub>OXA-23</sub> genes (oxacillinase genes) were dominant genes, but bla<sub>IMP</sub> and bla<sub>OXA-58</sub> were not local carbapenem resistant genes in Isfahan.</p>","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"17 1","pages":"392"},"PeriodicalIF":1.6000,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684280/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification of bla<sub>OXA-51-23-58</sub>, bla<sub>VIM</sub>, bla<sub>NDM</sub>, and bla<sub>IMP</sub> carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients.\",\"authors\":\"Kosar Salmani Nasrabadi, Yasaman Ahmadi, Seyed Mahdi Ghasemi, Dariush Shokri\",\"doi\":\"10.1186/s13104-024-07047-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.</p><p><strong>Results: </strong>In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting bla<sub>OXA-51</sub>, bla<sub>OXA-23</sub>, bla<sub>OXA-58</sub>, bla<sub>NDM</sub>, bla<sub>IMP</sub>, and bla<sub>VIM</sub> genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the bla<sub>OXA-23</sub> gene, 23 (92.0%) contained the bla<sub>OXA-51</sub> gene, and 1 (4.0%) isolate carried the bla<sub>NDM</sub> gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. bla<sub>OXA-51</sub> and bla<sub>OXA-23</sub> genes (oxacillinase genes) were dominant genes, but bla<sub>IMP</sub> and bla<sub>OXA-58</sub> were not local carbapenem resistant genes in Isfahan.</p>\",\"PeriodicalId\":9234,\"journal\":{\"name\":\"BMC Research Notes\",\"volume\":\"17 1\",\"pages\":\"392\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-12-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684280/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Research Notes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s13104-024-07047-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-024-07047-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
Identification of blaOXA-51-23-58, blaVIM, blaNDM, and blaIMP carbapenemase genes in Acinetobacter baumannii isolates from hospitalized patients.
Objective: The increase of multidrug-resistant (MDR) strains of Acinetobacter baumannii (A. baumannii), especially carbapenem-resistant strains, is challenging for treating infections. This study investigated the antibiotic resistance pattern and frequency of carbapenem resistance genes (oxacillinase and metallo-beta-lactamase) in A. baumannii.
Results: In this study, 100 bacterial isolates were collected from clinical samples from different hospitals in Isfahan, central of Iran. Of 100 samples of bloodstream, urine, cerebrospinal fluid (CSF), wound, and trachea, 60 bacteria were identified as A. baumannii. The results showed that 100% of the selected isolates were resistant to cefotaxime, ceftazidime, ciprofloxacin, piperacillin-tazobactam, and meropenem. Based on the antibiotic resistance pattern, 25 isolates were chosen for PCR analysis targeting blaOXA-51, blaOXA-23, blaOXA-58, blaNDM, blaIMP, and blaVIM genes PCR results revealed that among the selected isolates, 15 (60.0%) harbored the blaOXA-23 gene, 23 (92.0%) contained the blaOXA-51 gene, and 1 (4.0%) isolate carried the blaNDM gene. Based on MLST analysis, two colistin-resistant Acinetobacter baumannii isolates were categorized as ST2. The ST2 clone represents the predominant sequence type within the CC2 or international clone two. The results showed that the best antibiotic against isolates was colistin. blaOXA-51 and blaOXA-23 genes (oxacillinase genes) were dominant genes, but blaIMP and blaOXA-58 were not local carbapenem resistant genes in Isfahan.
BMC Research NotesBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.60
自引率
0.00%
发文量
363
审稿时长
15 weeks
期刊介绍：
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