Bruna Baccega, Juliana Montelli Fenalti, Alexia Brauner de Mello, Yan Wahast Islabão, Paloma T Birmann, Alessandra Neis, Marjorie de Giacometi, Francielle Liz Monteiro, Silvia de Oliveira Hubner, Rodrigo de Almeida Vaucher, Nara Amélia da Rosa Farias, Roghayeh Norouzi, Abolghasem Siyadatpanah, Camila Belmonte Oliveira
{"title":"柠檬醛和香叶醇对鸡毛滴虫的体外活性及细胞毒性研究。","authors":"Bruna Baccega, Juliana Montelli Fenalti, Alexia Brauner de Mello, Yan Wahast Islabão, Paloma T Birmann, Alessandra Neis, Marjorie de Giacometi, Francielle Liz Monteiro, Silvia de Oliveira Hubner, Rodrigo de Almeida Vaucher, Nara Amélia da Rosa Farias, Roghayeh Norouzi, Abolghasem Siyadatpanah, Camila Belmonte Oliveira","doi":"10.18502/ijpa.v19i4.17164","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against <i>Trichomonas gallinae</i> trophozoites.</p><p><strong>Methods: </strong>In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 10<sup>6</sup> parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween).</p><p><strong>Results: </strong>The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoites's viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for <i>T. gallinae</i> survival.</p><p><strong>Conclusion: </strong>The major compounds of lemongrass have potential antitrichomonal activity against <i>T. gallinae</i> in vitro.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"19 4","pages":"440-447"},"PeriodicalIF":0.9000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671819/pdf/","citationCount":"0","resultStr":"{\"title\":\"Properties of Compounds Citral and Geraniol on <i>Trichomonas gallinae</i>: Activity in Vitro and Cytotoxicity.\",\"authors\":\"Bruna Baccega, Juliana Montelli Fenalti, Alexia Brauner de Mello, Yan Wahast Islabão, Paloma T Birmann, Alessandra Neis, Marjorie de Giacometi, Francielle Liz Monteiro, Silvia de Oliveira Hubner, Rodrigo de Almeida Vaucher, Nara Amélia da Rosa Farias, Roghayeh Norouzi, Abolghasem Siyadatpanah, Camila Belmonte Oliveira\",\"doi\":\"10.18502/ijpa.v19i4.17164\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against <i>Trichomonas gallinae</i> trophozoites.</p><p><strong>Methods: </strong>In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 10<sup>6</sup> parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween).</p><p><strong>Results: </strong>The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoites's viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for <i>T. gallinae</i> survival.</p><p><strong>Conclusion: </strong>The major compounds of lemongrass have potential antitrichomonal activity against <i>T. gallinae</i> in vitro.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"19 4\",\"pages\":\"440-447\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671819/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v19i4.17164\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v19i4.17164","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Properties of Compounds Citral and Geraniol on Trichomonas gallinae: Activity in Vitro and Cytotoxicity.
Background: Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against Trichomonas gallinae trophozoites.
Methods: In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 106 parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween).
Results: The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoites's viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for T. gallinae survival.
Conclusion: The major compounds of lemongrass have potential antitrichomonal activity against T. gallinae in vitro.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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