用有限的蛋白水解结合质谱法探索蛋白质构象。

IF 11.6 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Trends in Biochemical Sciences Pub Date : 2025-02-01 DOI:10.1016/j.tibs.2024.11.005

Chloé Van Leene , Laura Van Moortel , Karolien De Bosscher , Kris Gevaert

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引用次数: 0

摘要

有限蛋白水解耦合质谱（LiP-MS）已成为研究蛋白质构象的强大蛋白质组学工具。自2014年推出以来，LiP-MS已将其范围扩大到探索复杂生物系统和揭示疾病机制，并已用于蛋白质药物研究。本文讨论了该技术的发展，最近的技术进步，包括增强的协议和机器学习的集成，以及各种实验模型的不同应用。尽管取得了一定的成就，但在蛋白质提取和构象肽鉴定方面仍然存在挑战。正在进行的方法改进将是克服这些挑战和提高技术能力的关键。然而，LiP-MS为未来结构蛋白质组学和医学研究的发现提供了巨大的潜力。

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Exploring protein conformations with limited proteolysis coupled to mass spectrometry

Limited proteolysis coupled to mass spectrometry (LiP-MS) has emerged as a powerful proteomic tool for studying protein conformations. Since its introduction in 2014, LiP-MS has expanded its scope to explore complex biological systems and shed light on disease mechanisms, and has been used for protein drug research. This review discusses the evolution of the technique, recent technical advances, including enhanced protocols and integration of machine learning, and diverse applications across various experimental models. Despite its achievements, challenges in protein extraction and conformotypic peptide identification remain. Ongoing methodological refinements will be crucial to overcome these challenges and enhance the capabilities of the technique. However, LiP-MS offers significant potential for future discoveries in structural proteomics and medical research.

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来源期刊

Trends in Biochemical Sciences 生物-生化与分子生物学

CiteScore

22.90

自引率

0.70%

发文量

148

审稿时长

6-12 weeks

期刊介绍： For over 40 years, Trends in Biochemical Sciences (TIBS) has been a leading publication keeping readers informed about recent advances in all areas of biochemistry and molecular biology. Through monthly, peer-reviewed issues, TIBS covers a wide range of topics, from traditional subjects like protein structure and function to emerging areas in signaling and metabolism. Articles are curated by the Editor and authored by top researchers in their fields, with a focus on moving beyond simple literature summaries to providing novel insights and perspectives. Each issue primarily features concise and timely Reviews and Opinions, supplemented by shorter articles including Spotlights, Forums, and Technology of the Month, as well as impactful pieces like Science & Society and Scientific Life articles.