多能干细胞高效生成人动脉和静脉内皮细胞的方法。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-03-21 Epub Date: 2024-12-19 DOI:10.1016/j.xpro.2024.103494

Kyle M Loh, Sherry Li Zheng, Kevin J Liu, Qingqing Yin, Zhainib A Amir-Ugokwe, Sawan K Jha, Yue Qi, Vanessa K Wazny, Alana T Nguyen, Angela Chen, Faith-Masong Njunkeng, Christine Cheung, Edda Spiekerkoetter, Kristy Red-Horse, Lay Teng Ang

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引用次数: 0

摘要

血管遍布所有器官，在健康和疾病中发挥着无数的作用。在这里，我们提出了一种在3-4天内从多能干细胞分化有效地产生人动脉和静脉内皮细胞（ECs）的方案。我们描述了如何播种人类多能干细胞，并依次将其分化为原始条纹、外侧中胚层和动脉或静脉内皮细胞。我们在明确的、无血清的单层培养基中分化干细胞，不需要饲养细胞或基因操作。关于该协议的使用和执行的完整细节，请参考Ang等人。

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Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells.

Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al. ¹.

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