{"title":"Recent Advances in DNA Origami-Enabled Optical Biosensors for Multi-Scenario Application.","authors":"Ziao Hao, Lijun Kong, Longfei Ruan, Zhengtao Deng","doi":"10.3390/nano14231968","DOIUrl":null,"url":null,"abstract":"<p><p>Over the past few years, significant progress has been made in DNA origami technology due to the unrivaled self-assembly properties of DNA molecules. As a highly programmable, addressable, and biocompatible nanomaterial, DNA origami has found widespread applications in biomedicine, such as cell scaffold construction, antimicrobial drug delivery, and supramolecular enzyme assembly. To expand the scope of DNA origami application scenarios, researchers have developed DNA origami structures capable of actively identifying and quantitatively reporting targets. Optical DNA origami biosensors are promising due to their fast-to-use, sensitive, and easy implementation. However, the conversion of DNA origami to optical biosensors is still in its infancy stage, and related strategies have not been systematically summarized, increasing the difficulty of guiding subsequent researchers. Therefore, this review focuses on the universal strategies that endow DNA origami with dynamic responsiveness from both de novo design and current DNA origami modification. Various applications of DNA origami biosensors are also discussed. Additionally, we highlight the advantages of DNA origami biosensors, which offer a single-molecule resolution and high signal-to-noise ratio as an alternative to traditional analytical techniques. We believe that over the next decade, researchers will continue to transform DNA origami into optical biosensors and explore their infinite possible uses.</p>","PeriodicalId":18966,"journal":{"name":"Nanomaterials","volume":"14 23","pages":""},"PeriodicalIF":4.4000,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomaterials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.3390/nano14231968","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
由于 DNA 分子具有无与伦比的自组装特性,DNA 折纸技术在过去几年中取得了重大进展。作为一种高度可编程、可寻址和生物兼容的纳米材料,DNA 折纸已在生物医学领域得到广泛应用,如细胞支架构建、抗菌药物输送和超分子酶组装等。为了扩大 DNA 折纸的应用范围,研究人员开发了能够主动识别和定量报告目标的 DNA 折纸结构。光学 DNA 折纸生物传感器具有使用快速、灵敏度高、易于实现等优点,因此前景广阔。然而,将 DNA 折纸转化为光学生物传感器的研究仍处于起步阶段,相关策略尚未得到系统总结,增加了指导后续研究人员的难度。因此,本综述重点从全新设计和现有的 DNA 折纸修饰两方面探讨赋予 DNA 折纸动态响应性的通用策略。文中还讨论了 DNA 折纸生物传感器的各种应用。此外,我们还强调了 DNA 折纸生物传感器的优势,它具有单分子分辨率和高信噪比,是传统分析技术的替代品。我们相信,在未来十年中,研究人员将继续把 DNA 折纸转化为光学生物传感器,并探索其无限可能的用途。
Recent Advances in DNA Origami-Enabled Optical Biosensors for Multi-Scenario Application.
Over the past few years, significant progress has been made in DNA origami technology due to the unrivaled self-assembly properties of DNA molecules. As a highly programmable, addressable, and biocompatible nanomaterial, DNA origami has found widespread applications in biomedicine, such as cell scaffold construction, antimicrobial drug delivery, and supramolecular enzyme assembly. To expand the scope of DNA origami application scenarios, researchers have developed DNA origami structures capable of actively identifying and quantitatively reporting targets. Optical DNA origami biosensors are promising due to their fast-to-use, sensitive, and easy implementation. However, the conversion of DNA origami to optical biosensors is still in its infancy stage, and related strategies have not been systematically summarized, increasing the difficulty of guiding subsequent researchers. Therefore, this review focuses on the universal strategies that endow DNA origami with dynamic responsiveness from both de novo design and current DNA origami modification. Various applications of DNA origami biosensors are also discussed. Additionally, we highlight the advantages of DNA origami biosensors, which offer a single-molecule resolution and high signal-to-noise ratio as an alternative to traditional analytical techniques. We believe that over the next decade, researchers will continue to transform DNA origami into optical biosensors and explore their infinite possible uses.
期刊介绍:
Nanomaterials (ISSN 2076-4991) is an international and interdisciplinary scholarly open access journal. It publishes reviews, regular research papers, communications, and short notes that are relevant to any field of study that involves nanomaterials, with respect to their science and application. Thus, theoretical and experimental articles will be accepted, along with articles that deal with the synthesis and use of nanomaterials. Articles that synthesize information from multiple fields, and which place discoveries within a broader context, will be preferred. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental or methodical details, or both, must be provided for research articles. Computed data or files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Nanomaterials is dedicated to a high scientific standard. All manuscripts undergo a rigorous reviewing process and decisions are based on the recommendations of independent reviewers.