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IF 2.7 2区医学 Q2 PATHOLOGY

Human pathology Pub Date : 2024-12-15 DOI:10.1016/j.humpath.2024.105707

Dena Khaefpanah, Somaye Zare, Farnaz Hasteh, Wangpan J Shi, Omonigho Aisagbonhi, Andres A Roma, Oluwole Fadare

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引用次数: 0

摘要

胃[肠]型子宫内膜粘液癌（MCG）是一种罕见的、可能具有侵袭性的子宫内膜癌亚型，应将其与子宫内膜样癌（EEC）等潜在的拟态癌区分开来。在此，我们评估了无明显胃/胃肠道型形态的 EEC 中胃和胃肠道免疫表型的频率。我们对 81 例 EEC 进行了 KRT(CK)7、KRT20、CDX2、ER、SATB2、MUC6、PAX8 和 HIK1083 的免疫组化分析，其中包括连续存档的低级别病例（有（22 例）和无（47 例）粘液分化）和高级别病例（12 例）。所有病例均未显示胃型形态或小腺细胞。表达水平以标准化的 0-300 级 H 分数（结合染色强度和范围）进行半量化。在胃/胃肠型标志物中，分别有56%、62%、23%、25%和0%的病例表达MUC6、CDX2、KRT20、SATB2和HIK1083。阳性病例的表达水平普遍较低，MUC6 的平均 H 分数为 49.5 [范围 1-250]，CDX2 为 33.7 [1-285]，CK20 为 24.0 [1-270]，SATB2 为 30.5 [2-220]。10个病例（12.35%）至少有1个胃/胃肠型标志物呈高表达（H≥200），其中KRT20、SATB2、CDX2和MUC6分别有1、2、2和6个高阳性。在形态学层面上，免疫反应灶一般与背景无异。任何胃/胃肠型标志物的表达与ER、KRT7或PAX8的表达之间都没有统计学意义上的相关性。总之，胃/胃肠型蛋白在 EEC 中的低水平表达并不少见。因此，这些标记物的阳性不能作为区分 EEC 和 MCG 的唯一依据。

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Characterization of gastric/gastrointestinal-like immunophenotypes in endometrial endometrioid adenocarcinomas, including endometrioid adenocarcinomas with mucinous differentiation.

Endometrial mucinous carcinoma of the gastric [gastrointestinal] type (MCG) is a rare, possibly aggressive subtype of endometrial cancer that should be distinguished from its potential mimics, including endometrioid carcinoma (EEC). Herein, we assess the frequency of gastric and gastrointestinal immunophenotypes in EEC without any discernible gastric/gastrointestinal-type morphology. Immunohistochemical analyses for KRT(CK)7, KRT20, CDX2, ER, SATB2, MUC6, PAX8, and HIK1083 were performed on 81 EEC, inclusive of consecutively archived low grade [with (n = 22) and without (n = 47) mucinous differentiation] and high grade (n = 12) cases. None displayed gastric-type morphology or goblet cells. Expression levels were semi-quantified as H-scores (combining intensity and extent of staining) on a standardized 0-300 scale. Among the gastric/gastrointestinal-type markers, 56%, 62%, 23%, 25%, and 0% of cases expressed MUC6, CDX2, KRT20, SATB2, and HIK1083 respectively. The expression levels for positive cases were generally limited, with average H-scores being 49.5 [range 1-250] for MUC6, 33.7 [1-285] for CDX2, 24.0 [1-270] for CK20, and 30.5 [2-220] for SATB2. Ten (12.35%) cases showed high expression (H ≥ 200) of at least 1 gastric/gastrointestinal-type marker, including 1, 2, 2 and 6 cases that were high positive for KRT20, SATB2, CDX2 and MUC6 respectively. Immunoreactive foci were generally indistinguishable from background at the morphologic level. There was no statistically significant correlation between the expression of any of the gastric/gastrointestinal-type markers and ER, KRT7 or PAX8 expression. In summary, gastric/gastrointestinal-type proteins are not uncommonly expressed at low levels in EEC. As such, positivity for these markers cannot be the sole basis for distinguishing EEC from MCG.

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来源期刊

Human pathology 医学-病理学

CiteScore

5.30

自引率

6.10%

发文量

206

审稿时长

21 days

期刊介绍： Human Pathology is designed to bring information of clinicopathologic significance to human disease to the laboratory and clinical physician. It presents information drawn from morphologic and clinical laboratory studies with direct relevance to the understanding of human diseases. Papers published concern morphologic and clinicopathologic observations, reviews of diseases, analyses of problems in pathology, significant collections of case material and advances in concepts or techniques of value in the analysis and diagnosis of disease. Theoretical and experimental pathology and molecular biology pertinent to human disease are included. This critical journal is well illustrated with exceptional reproductions of photomicrographs and microscopic anatomy.