揭示种子隐藏的复杂性:利用受激拉曼散射显微镜对种子涂层进行化学选择性成像

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Analyst Pub Date : 2024-12-17 DOI:10.1039/d4an01409j
Chun-Chin Wang, Steven Penfield, julian moger
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引用次数: 0

摘要

种皮在种子的发育和萌发过程中起着至关重要的作用,它是种子的保护屏障,也是种子与外界环境相互作用的媒介。传统的组织化学技术和分析方法为了解种皮的组成和功能提供了宝贵的信息。然而,这些方法往往受到间接化学特征和缺乏空间分辨率等限制。在这里,我们将受激拉曼散射(SRS)显微镜作为一种新型分析工具,用于无损、无标记、高分辨率地绘制完整种皮中的生物聚合物、水和应用活性成分(AIs)。我们展示了 SRS 显微镜对主要种皮生物聚合物(果胶、单宁和单宁酸)进行深度分辨和化学选择性成像的能力。通过比较野生型拟南芥种子与缺乏单宁酸和单宁的转基因突变体,我们展示了对生物聚合物含量进行半定量分析的潜力。此外,我们还利用氘化水(D2O)吸收研究表明,SRS 显微镜可以跟踪种皮对水的渗透性。实时成像揭示了野生型种子和小檗苷缺乏型种子在透水性方面的差异,突出了种皮成分在萌发过程中调节水分吸收的重要性。此外,我们还将 SRS 显微技术的应用范围扩大到了大型种子,如黄花菜,利用外延检测成像技术进行表面研究。最后,我们使用一种氚代杀虫剂(氯噻菌胺-d3)展示了 SRS 显微镜观察人工合成物质融入种皮的能力。我们的研究表明,SRS 显微镜是表征种皮成分和了解低分子量化合物向种子扩散的有力工具。这项技术为设计具有定制特性的种子提供了新的机遇,从而提高种子的萌发率和对环境胁迫的适应能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Shedding New Light on the Hidden Complexity of Seeds: Chemically Selective Imaging of Seed Coats with Stimulated Raman Scattering Microscopy
The seed coat plays a pivotal role in seed development and germination, acting as a protective barrier and mediating interac-tions with the external environment. Traditional histochemical techniques and analytical methods have provided valuable insights into seed coat composition and function. However, these methods often suffer from limitations such as indirect chemical signatures and lack of spatial resolution. Here, we introduce stimulated Raman scattering (SRS) microscopy as a novel analytical tool for non-destructive, label-free, high-resolution mapping of biopolymers, water and applied active ingre-dients (AIs) in intact seed coats. We demonstrate the capability of SRS microscopy to perform depth-resolved, chemically selective imaging of major seed coat biopolymers (pectin, tannin, and suberin). By comparing wild type arabidopsis thali-ana seeds with genetically modified mutants deficient in suberin and tannin, we illustrate the potential for semi-quantitative analysis of biopolymer content. Furthermore, we show that SRS microscopy can track the permeability of seed coats to wa-ter using deuterated water (D2O) uptake studies. Real-time imaging reveals differences in water permeation between wild type and suberin deficient seeds, highlighting the importance of seed coat composition in regulating water uptake during germina-tion. Additionally, we extend the application of SRS microscopy to large seeds, such as brassica oleracea, utilizing epi-detected imaging for surface studies. Finally, using a deuterated insecticide (clothianidin-d3), we demonstrate the capability of SRS microscopy to visualize the incorporation of AIs into seed coats. Our study presents SRS microscopy as a powerful tool for characterizing seed coat composition and understanding the diffusion of low molecular weight compounds into seeds. This technique offers new opportunities for designing seeds with tailored properties for improved germination and resilience to environmental stressors.
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来源期刊
Analyst
Analyst 化学-分析化学
CiteScore
7.80
自引率
4.80%
发文量
636
审稿时长
1.9 months
期刊介绍: "Analyst" journal is the home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences.
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