IF 4.5 3区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY
Risa Nakanishi, Takeshi Kuwada, Masahiro Shiokawa, Yoshihiro Nishikawa, Sakiko Ota, Hajime Yamazaki, Takafumi Yanaidani, Kenji Sawada, Ayako Hirata, Muneji Yasuda, Ikuhisa Takimoto, Koki Chikugo, Masataka Yokode, Yuya Muramoto, Shimpei Matsumoto, Tomoaki Matsumori, Norimitsu Uza, Tsutomu Chiba, Hiroshi Seno
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引用次数: 0

摘要

背景:肠袋炎是溃疡性结肠炎(UC)患者进行回肠肠袋-肛门吻合术(IPAA)的恢复性直肠切除术(RPC)最常见的并发症。我们曾报道过 UC 患者血清中存在抗整合素 αvβ6 抗体。本研究探讨了抗整合素αvβ6抗体与溃疡性结肠炎患者发生储袋炎之间的关系:通过酶联免疫吸附试验检测了 16 名接受 RPC 和 IPAA 的 UC 患者血清中的抗整合素 αvβ6 抗体水平。采用免疫组化和免疫印迹分析法检测了整合素αvβ6在结肠、回肠末端和肠袋上皮细胞中的表达:结果:RPC 后 3 个月、9 个月和 12 个月,UC 患者的抗整合素 αvβ6 抗体水平显著下降(PUC 患者的抗整合素 αvβ6 抗体水平在 RPC 后有所下降,但在发生袋炎的患者中仍然很高。RPC 时的抗体水平可作为预测 UC 患者发生袋炎风险的潜在预后生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-integrin αvβ6 Antibodies Predict Pouchitis in Patients With Ulcerative Colitis After Restorative Proctocolectomy With Ileal Pouch-Anal Anastomosis.

Background: Pouchitis is the most common complication of restorative proctocolectomy (RPC) with ileal pouch-anal anastomosis (IPAA) in patients with ulcerative colitis (UC). We previously reported the presence of anti-integrin αvβ6 antibodies in the serum of patients with UC. This study investigated the association between anti-integrin αvβ6 antibodies and the development of pouchitis in patients with UC.

Methods: Serum levels of anti-integrin αvβ6 antibodies were measured by enzyme-linked immunosorbent assay in 16 patients with UC who underwent RPC with IPAA. Integrin αvβ6 expression in the colonic, terminal ileal, and pouch epithelium was examined using immunohistochemistry and western blot analysis.

Results: Anti-integrin αvβ6 antibody levels in patients with UC were significantly decreased at 3, 9, and 12 months after RPC (P < .05). However, in patients who developed pouchitis, antibody levels remained high. The antibody levels at the time of RPC were significantly higher in patients who developed pouchitis compared to those who did not. Kaplan-Meier analysis revealed a significantly higher incidence of pouchitis in patients with antibody levels above the cutoff at the time of RPC. Although integrin αvβ6 was not expressed in the terminal ileal epithelium at the time of RPC, expression became positive in the pouch epithelium of patients with pouchitis.

Conclusions: The anti-integrin αvβ6 antibody levels in patients with UC were decreased after RPC but remained high in patients who developed pouchitis. The antibody levels at the time of RPC may serve as a potential prognostic biomarker for predicting the risk of pouchitis in patients with UC.

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来源期刊
Inflammatory Bowel Diseases
Inflammatory Bowel Diseases 医学-胃肠肝病学
CiteScore
9.70
自引率
6.10%
发文量
462
审稿时长
1 months
期刊介绍: Inflammatory Bowel Diseases® supports the mission of the Crohn''s & Colitis Foundation by bringing the most impactful and cutting edge clinical topics and research findings related to inflammatory bowel diseases to clinicians and researchers working in IBD and related fields. The Journal is committed to publishing on innovative topics that influence the future of clinical care, treatment, and research.
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